Objective: To study the intergenus protoplast fusion bioactive marine Streptomyces sp ANS4 with the Escherichia coli for the strain improvement. Methods: Protoplast of both the organisms was prepared by lysozyme treatment and fused using polyethylene glycol (PEG). Fusant colonies were regenerated using regeneration medium where all the regenerated colonies are powdery in consistency. Microscopi...

Protoplast fusion was investigated as a technique for genetically manipulating two lignin-degrading Streptomyces strains, Streptomyces viridosporus T7A and Streptomyces setonii 75Vi2. Four of 19 recombinants tested showed enhanced production of acid-precipitable polymeric lignin (APPL), producing 155 to 264% more APPL from corn stover lignocellulose than was produced by the wild-type S. viridos...

The construction of high-yield laccase-producing fusant was achieved by inter-kingdom protoplast fusion between Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditions demonstrated that with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants were screened by different characters between two parental strai...

The date palm has a pivotal role in the oasis ecosystem and it is an important food crop and commercial commodity. The cloning or protoplast fusion can increase breeding and multiplication of many varieties. Present investigation has been undertaken with the aim to develop methods for obtaining plantlets by somatic embryogenesis either from vegetative or female floral explants, as well as by th...

Protoplasts were isolated from five species of Gossypium. · Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated from cell suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. k/otzschianum, G. harlmessii, and G. herbaceum. Protop...