Sour cherry cv. Marasca is considered a native Croatian variety with the best fruit quality in Northern Dalmatia region. The current production not sufficient to cover demand, therefore new orchards should be established pathogen-tested planting material. To select virus- and bacteria-free mother trees, survey was conducted largest plantation "Vlačine". ELISA on 205 trees (51 elite, 103 average...

Prunus necrotic ringspot virus (PNRSV) and cherry A (CVA) are two viruses that mainly infect plants of the genus Prunus. Full-length sequences these viruses, collected in Czech Republic from cerasifera plants, were obtained via HTS sequencing. Phylogenetic analyses based on NJ method Splitstree tools showed PNRSV isolate (ON088600-ON088602) is a divergent other molecular groups, sharing less th...

The movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is required for viral transport. Previous analysis with MPs of other members of the family Bromoviridae has shown that the C-terminal part of these MPs plays a critical role in the interaction with the cognate coat protein (CP) and in cell-to-cell transport. Bimolecular fluorescence complementation and overlay analysis confirm ...

RNA silencing is a powerful technology for molecular characterization of gene functions in plants. A commonly used approach to the induction of RNA silencing is through genetic transformation. A potent alternative is to use a modified viral vector for virus-induced gene silencing (VIGS) to degrade RNA molecules sharing similar nucleotide sequence. Unfortunately, genomic studies in many allogamo...

The virulence factor of Melon necrotic spot virus (MNSV), a virus that induces systemic necrotic spot disease on melon plants, was investigated. When the replication protein p29 was expressed in N. benthamiana using a Cucumber mosaic virus vector, necrotic spots appeared on the leaf tissue. Transmission electron microscopy revealed abnormal mitochondrial aggregation in these tissues. Fractionat...

Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcripti...

PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV) from 53 PNRSV-infected trees using amplicons from conserved g...