background: varicella zoster virus (vzv) causes chickenpox in children and zoster (zona) in the elderly. using rflp-pcr method for detection of vzv specific snps orf38, 54 and 62 could  distinguish the profile of vzv isolates. the aim of this study was to investigate enzymatic digestion pattern of vzv orf38 and orf54 in chickenpox patients using rflp technique. methods: thirty-eight chickenpox ...

PURPOSE To compare the efficacy of posterior sub-Tenon's capsule triamcinolone acetonide injection combined with modified grid macular photocoagulation (PSTI + MP) with intravitreal triamcinolone acetonide (IVTA) injection in the treatment of diffuse diabetic macular edema (DME). MATERIALS AND METHODS Forty eyes of 33 patients with diffuse DME were randomly allocated into either PSTI + MP (20...

BACKGROUND Varicella zoster virus (VZV) causes chickenpox in children and zoster (zona) in the elderly. Using RFLP-PCR method for detection of VZV specific SNPs ORF38, 54 and 62 could distinguish the profile of VZV isolates. The aim of this study was to investigate enzymatic digestion pattern of VZV ORF38 and ORF54 in chickenpox patients using RFLP technique. METHODS Thirty-eight chickenpox p...

INTRODUCTION Genotyping of wild type of varicella zoster virus (VZV) in Sri Lanka would help to distinguish the VZV wild type infection from varicella vaccine associated infections. METHODS PCR-RFLP analysis of VZV ORF 38, 54 and 62 was used for genotyping in VZV from blood or vesicular fluid from 31 patients with chickenpox or herpes zoster. The PstI restriction site of ORF 38, BglI restrict...

Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, stained with Giemsa, and examined for banding patterns. The following species were studied: Crassos...

BACKGROUND Previous reports implicate CYP2E1 RsaI/PstI polymorphism as a possible risk factor for several cancers. Published studies on the relationship of CYP2E1 RsaI/PstI polymorphisms with the susceptibility to gastric cancer are controversial. This study aimed to determine this relationship accurately. METHODS Meta-analyses that assessed the association of CYP2E1 RsaI/PstI variations with...

Parkinson's disease (PD) is one of the most common progressive neurodegenerative disorders, characterized by resting tremor, rigidity, bradykinesia, and postural instability. These symptoms are associated with massive loss of tyrosine hydroxylase-positive neurons in the substantia nigra (SN) causing an estimated 70-80% depletion of dopamine (DA) in the striatum, where their projections are loca...

BACKGROUND Cytochrome P450 2E1 (CYP2E1) might be involved in the development of bladder cancer. However, previous studies of any association between CYP2E1 RsaI/PstI polymorphism and bladder cancer risk have yielded conflicting results. In this study, we performed a more precise estimation of the relationship by a meta-analysis based on the currently available evidence from the literature. ME...

Pancreatic secretory trypsin inhibitor (PSTI), a specific trypsin inhibitor secreted by acinar cells of pancreas, also acts as a growth factor. PSTI in various cancers has been reported to be associated with tumor invasiveness and pathological malignancy; however, such studies in urological cancers have not yet been performed. We therefore examined the expression of PSTI in fresh frozen specime...

Background: North of Iran is amongst high incidence rate areas of gastric carcinoma where environmental carcinogenic compounds especially agricultural pesticides are massively used. Cytochrome P450 2E1 (CYP2E1) enzyme metabolically activates a large number of low molecular mass xenobiotics. The polymorphic nature of cyp2E1 gene control elements is associated with interindividual differences...