OBJECTIVES To investigate the time course of a monoarthritis induced with the purified protein derivative of tuberculin (PPD) or a recombinant 65 kDa heat shock protein (rhsp65) in two different strains of sensitised rats. METHODS Wistar and Lewis rats, sensitised with heat killed Mycobacterium tuberculosis in oil, were challenged intraarticularly with PPD or rhsp65 and monitored for six week...

The T-helper (Th) 1 T-cell response to purified protein derivative (PPD) is known to be suppressed in tuberculosis patients which favours intracellular survival of the bacilli. We demonstrate that the Mycobacterium tuberculosis heat shock protein 60 (Mtbhsp60) plays an important role to skew the anti-PPD T-cell response towards the Th2 type when macrophages were used as antigen presenting cells...

After Mantoux testing with purified protein derivative (PPD) equivalent to 5 tuberculin units (TU), a 28 yr old man developed extensive erythema, induration and proximal lymphangitis. This is the only such case observed after Mantoux testing in approximately 500,000 subjects during 10 yrs in the Greek Army.

DESCRIPTION Tuberculin Purified Protein Derivative (Mantoux) TUBERSOL for intradermal (Mantoux) tuberculin testing is available in a stabilized solution bio-equivalent to 5 U.S. units (TU) PPD-S per test dose (0.1 mL). TUBERSOL is prepared by Aventis Pasteur Limited from a large Master Batch, Connaught Tuberculin (CT68) and is a cell-free purified protein fraction obtained from a human strain o...

Purified protein derivative (PPD) tuberculin induced immunoglobulin production in cultures of nonimmune mouse spleen cells, as measured by plaque-forming cells (PFC) against sheep erythrocytes (SRBC), horse erythrocytes, and 4-hydroxy-3,5-dinitrophenacetyl-SRBC. The increase started between 15 and 20 h of culture and reached a peak at 48-72 h. Higher PPD concentrations resulted in earlier peak ...

The functional properties of lymphocytes in pleural fluid were studied in 23 patients admitted to the hospital for the diagnostic evaluation of a unilateral or bilateral pleural effusion. The in vitro reactivities of the patients' pleural fluid lymphocytes and peripheral blood lymphocytes to phytohemagglutinin (PHA) and to purified protein derivative (PPD) were compared. In most patients with p...

The Delayed type hypersensitivity skin test (DTH) and interferon-gamma assay are used for the diagnosis of bovine tuberculosis (TBB). The specificity of these diagnoses, however, is compromised because both are based on the response against purified protein derivative of Mycobacterium bovis (PPD-B). In this study, we assessed the potential of two cocktails containing M. bovis recombinant protei...

Formation of pyropheophorbide (PyroPheid) during chlorophyll metabolism in some higher plants has been shown to involve the enzyme pheophorbidase (PPD). This enzyme catalyzes the conversion of pheophorbide (Pheid) a to a precursor of PyroPheid, C-13(2)-carboxylPyroPheid a, by demethylation, and then the precursor is decarboxylated non-enzymatically to yield PyroPheid a. In this study, expressio...