A combination of purines and thymine has long been known to substitute for folic acid (FA) in the nutrition of microorganisms requiring the vitamin. The purines were found to be interchangeable, but the effect of thymine was specific and could only be duplicated by its desoxyriboside, thymidine (l-4). From these and other studies on inhibition analysis (5, S), it was concluded that the purines ...

Measurement of ruminal microbial protein is necessary to quantify ruminal escape of dietary protein and microbial yields. Microbial markers used most widely have been the internal markers, diaminopimelic acid and nucleic acids (RNA, DNA, individual purines and pyrimidines, or total purines), and the external isotopic markers (e.g., 15N and 35S). Combined with digesta flow markers in ruminally a...

Objective: Adenosine 5'-triphosphate (ATP) is released from the bladder mucosa during bladder filling and is assumed to stimulate sensory neurons in the lamina propria and to regulate the apical delivery of fusiform vesicles in urothelial umbrella cells. As a parasympathetic cotransmitter, ATP contracts the detrusor smooth muscle. Therefore, extracellular ATP likely regulates the two phases of ...

Purines and pyrimidines are indispensable molecules of life; they fundamental for genetic code bioenergetics. From the very early evolution life purines have acquired meaning damage-associated extracellular signaller purinergic receptors emerged in unicellular organisms. Ancestral purinoceptors P2X-like ionotropic ligand-gated cationic channels showing 20–40% homology with vertebrate P2X recept...

haloalkyl purines i.e. 2,6-diamino-8-(chloromethyl) purine 10; 2, 6-diamino-x- (chloromethyl) purine 17; 2,6-diamino-8-(chloropropylp) urine 18; were synthesized by two distinct but facile methods. the n-alkylating potential of 2, 6-diamino-8- (chloromethyl) purine was investigated. the following products 2,6-diamino-8-(nbenzyl- n-methyl) purine 11; 2,6-diamino-8-(4-nitro anilinomethyl) purine ...