1. Evidence is presented for the presence of pyridoxamine phosphate oxidase and a pyridoxal phosphate phosphatase in aqueous extracts of E8cherichia coli. 2. Spectrophotometric methods have been developed for the precise measurement of substrate and product concentrations in both enzyme systems. Specific activities of the order of 10-2 tumole/30 min./mg. ofprotein/ml. ofreaction mixture are rep...

The combination of pyridoxamine 5-phosphate with the apoprotein of the enzyme aspartate aminotransferase has been followed by measuring the quenching of protein fluorescence associated with complex formation. The reaction takes place in two steps; an initial rapid decrease in protein fluorescence is followed by a slow quenching process which parallels the recovery of catalytic activity. Similar...

The general subject of enzymatic transamination has been extensively reviewed by several workers (Braunstein (I) ; Snell (2) ; Meister (3)). Glutamic-aspartic transaminase (L-aspartate : 2-oxoglutarate aminotransferase, EC 2.6.1.1) from pig heart. has been prepared by Jenkins, Yphantis, and Sizer (4) and shown to contain 2 molecules of pyridoxal phosphate per enzyme molecule of molecular weight...

The stability constants of species present in the systems Ni(II)-pyridoxamine(pym)(A) and Ni(II)-pyridoxamine(pym)(A)-imidazole containing ligands(B) [B = imidazole(him),  benzimidazole(bim), histamine(hist) and L-histidine(his)] have been determined pH-metrically using the MINIQUAD computer program. The existence of the species NiAH, NiA and NiA2 was proven for the Ni(II)-pym(A)...

[structure: see text] Pyridoxamine is known to be an effective inhibitor of both advanced glycation (AGE) and advanced lipoxidation (ALE) end products. The synthesis of a novel multifunctional AGE and ALE inhibitor, 6-dimethylaminopyridoxamine (dmaPM, 11) is described. The 6-dimethylamino substituent increases the radical trapping ability of pyridoxamine's phenolic group. Results obtained durin...

The general subject of enzymatic transamination has been extensively reviewed by several workers (Braunstein (I) ; Snell (2) ; Meister (3)). Glutamic-aspartic transaminase (L-aspartate : 2-oxoglutarate aminotransferase, EC 2.6.1.1) from pig heart. has been prepared by Jenkins, Yphantis, and Sizer (4) and shown to contain 2 molecules of pyridoxal phosphate per enzyme molecule of molecular weight...

the stability constants of species present in the systems ni(ii)-pyridoxamine(pym)(a) and ni(ii)-pyridoxamine(pym)(a)-imidazole containing ligands(b) [b = imidazole(him),  benzimidazole(bim), histamine(hist) and l-histidine(his)] have been determined ph-metrically using the miniquad computer program. the existence of the species niah, nia and nia2 was proven for the ni(ii)-pym(a) system, wherea...

Chlorine is a widely used disinfectant and oxidant for an array of municipal industrial applications, including potable water, swimming pools, cleaning membranes. The most popular method to verify the concentration free chlorine colorimetric based on DPD (N, N-diethyl-p-phenylenediamine), which fast reasonably cheap, but its product are potentially toxic. Therefore, novel, environmentally frien...