There is considerable variation in ways of preparing Nessler’s reagent as well as in the manner of forming the colored ammonobasic mercuric iodide. Two critical values are the ratio of KI to Hg19, i.e., x in HgL.xKI, and the final concentration of mercury. The value x determines the stability of solutions and to some extent the relative sensitivity to NH3. Vanselow (2) concluded that x should b...

The presence of insulin-binding antibodies in the serums of insulin-treated subjects has been reported previously (1). With I"ll-labeled insulin it has been shown that the antigen-antibody complexes do not precipitate, that the binding of insulin to antibody is a reversible process, and that, at constant antibody concentration, the ratio of bound insulin to free insulin is an inverse function o...

1. Antisera to R-salt-azo-benzidine-azo-crystalline egg albumin give precipitates with crystalline egg albumin by virtue of their antidye content. 2. The quantitative course of the reactions with increasing amounts of antigen is very similar for the dye-antidye and egg albumin-anti-egg albumin systems, but differs markedly for the cross reaction between egg albumin and antidye. 3. A possible ex...

1. The reaction between crystalline horse serum albumin and homologous antibody in rabbit sera is quantitatively accounted for by expressions similar to those derived from the law of mass action for other immune precipitating systems. 2. The reaction of an azo dye prepared from crystalline serum albumin by coupling with diazotized R-salt-azo benzidine was also studied with homologous antibody a...

As a promising tool for dissecting the genetic basis of common diseases, expression quantitative trait loci (eQTL) study has attracted increasing research interest. The traditional eQTL methods focus on testing the associations between individual single-nucleotide polymorphisms (SNPs) and gene expression traits. A major drawback of this approach is that it cannot model the joint effect of a set...