We developed a new in vitro assay for oligosaccharyltransferase (OST), which catalyzes the transfer of preassembled oligosaccharides on lipid carriers onto asparagine residues in polypeptide chains. The asparagine residues reside in the sequon, Asn-X-Thr/Ser, where X can be any amino acid residue except Pro. We demonstrate the potency of our assay using the OST from yeast. In our method, polyac...

References 1. Veit J. Untersuchungen Ober den osmotischen Druck zwischen Mutter und Kind. Z Geburtshilfe Gynaekol 1900;42:316-29. 2. Robertson GL. Thirst and vasopressin function in normal and disordered states of water metabolism. J Lab Clin Med 1983;101: 351-71. 3. Robertson GL. Osmoregulation ofthirst and vasopressin secretion: functional properties and their relationship to water balance. I...

The new technique of molecular imprinting has increasingly been adopted by research laboratories worldwide during the last few years. We have studied the use of such imprints against drugs as artificial antibody-binding mimics in competitive radioimmuno-style binding assays. The recognition sites "molded" in the polymers mimic the binding sites of natural antibodies in their interactions with t...

In vivo microdialysis coupled with HPLC and radioimmuno-assay techniques were used to analyze dopamine (DA) and neurotensin (NT) in prefrontal cortical extracellular fluid following electrical stimulation of mesocortical axons. The release (overflow into the extracellular fluid) of both DA and NT increased with increasing number of impulses and with frequency. At the lowest frequency tested (2....

The pharmacokinetics and the prostaglandin (PG) synthesis inhibiting effect of flunixin were determined in 6 Norwegian dairy goats. The dose was 2.2 mg/kg body weight administered by intravenous (i.v.). intramuscular (i.m.) and oral (p.o.) routes using a cross-over design. Plasma flunixin content was analysed by use of liquid chromatography and the PG synthesis was evaluated by measuring plasma...

Abstract Since Zirconium-89 ( 89 Zr or Zr-89) decays by emitting positron with a half-life of 78.4 hours, it has been suggested as diagnostic radioisotope for cancer. Normally, is generated irradiating an enriched yttrium-89 Y Y-89) target protons via Y(p,n) nuclear reaction. Optimum proton energy employed to produce high Zr-89 yield and low radioactive impurities required be determined so that...

In traditional practice medicine, plants are used in many countries to control diabetes mellitus. The hypoglycaemic actions of these medicinal plants are beings studied. Fenugreek (Trigonella foenum graecum) has been documented as a traditional treatment of diabetes. In the present study, oral administration of alcoholic fenugreek extract (0.1, 0.25, 0.5 g/kg body wt.) and glibenclamide (600 mg...