Hematopoietic stem cells (HSCs) are defined in mice by three activities: they must rescue lethally irradiated mice (radioprotection), they must self-renew, and they must restore all blood cell lineages permanently. We initially demonstrated that HSCs were contained in a rare ('~0.05%) subset of bone marrow cells with the following surface marker profile: Thy-l.1 l~ LinSca-l+. These cells were c...

Hematopoietic stem cells (HSCs) are defined in mice by three activities: they must rescue lethally irradiated mice (radioprotection), they must self-renew, and they must restore all blood cell lineages permanently. We initially demonstrated that HSCs were contained in a rare (approximately 0.05%) subset of bone marrow cells with the following surface marker profile: Thy-1.1lo Lin- Sca-1+. These...

It was previously shown on heat shock protein (Hsp)-overexpressing cell lines that the increased intracellular content of Hsp70 or Hsp27 is associated with the elevated radioresistance. However, it was so far unknown whether the in vivo Hsp induction by stressful preconditioning can confer radioprotection at the tissue and cellular levels. In the present study, we examined how the in vivo up-re...

The plasmid/liposome (PL) delivery of the manganese superoxide dismutase (MnSOD) transgene to lung, esophagus, oral cavity, bladder, or delivery of the same transgene by viral vectors including Herpes virus, adenovirus, and retrovirus to cells in culture or to intestine have been demonstrated to confer cellular and tissue resistance to ionizing iradiation damage including apoptosis. In contrast...

Ionising radiation (IR) is responsible for various types of tissue injury leading to morbidity at low doses and mortality at high radiation exposure. Although many radioprotective and pharmacological agents are being tested for decreasing radiation injury, however, the availability of Amifostine as the only clinically used radioprotector with limited indication has prompted us to find out new p...

Certifying professionals in radioprotection (RP), according to the requirements and qualifications established current Brazilian legislation, ensures that procedures practices radiology, radiotherapy nuclear medicine be performed based on fundamental principles of radioprotection. However, none regulations resolutions country involving use ionizing radiation, qualification RP for interventional...