A monoclonal antibody VCD-1, directed against the N-terminal intracellular part of the invariant chain (li) was used to show, by immunoprecipitation and Western blotting, the unprocessed and processed forms of li in chronic lymphocytic leukemia (CLL) cells, in Epstein-Barr virus-transformed normal lymphocytes (EBVL), and in cells of the Raji Burkitt's lymphoma cell line. Terminal glycosylation ...

at 37°C of IgG from the sera of 16 patients with systemic lupus erythematosus (SLE) to pronasedigested Raji cells was reduced much less consistently and extensively (9-100% reduction; mean reduction of 51%). In more detailed studies of two SLE sera, sucrose density gradient centrifugation showed that >50% of the IgG binding to undigested Raji cells sedimented in the 7S region. Pepsin digestion ...

The association of multidrug resistance (MDR) with resistance to lysis by natural killer (NK) and lymphokine-activated killer (LAK) cells is controversial. To address this issue further, drug-resistant variants of a human Burkitt lymphoma cell line (RAJI) were developed in vitro by intermittent exposure to Adriamycin® (R/ADR) or to etoposide (R/VP-16). The RAJI cell line was selected because it...

Epstein-Barr virus obtained by superinfection of Raji cells with Epstein-Barr virus recovered from P3HR1 cells (HRI virus) transformed human lymphocytes, but it did not superinfect Raji cells. A human lymphoblastoid cell line, HLB, established by such transformation contained 22 Epstein-Barr virus genomes per cell and Epstein-Barr virus-associated nuclear antigen, and a few cells contained earl...

The human immunodeficiency virus type I (HIV-1) regulatory gene, tat III, is a powerful trans-activator of gene expression from the viral long terminal repeat and is essential for HIV replication. In addition, tat III protein has been shown to be immunosuppressive as indicated by the inhibition of antigen mediated T-cell proliferation. To further test whether tat III might play a direct role in...

High-molecular-weight DNA from cell line Raji (derived from Burkitt's lymphoma), which contains 50-60 copies of Epstein-Barr virus DNA per cell, was fractionated in neutral solution by several cycles of CsCl gradient centrifugation in fixed-angle rotors. Under the fractionation conditions used, intact Epstein-Barr virus DNA from virus particles can be separated from the less-dense cellular DNA....

Raji, a human lymphoblastoid cell line, expresses a membrane receptor (EBVR) specific for Epstein Barr virus (EBV). A component that binds EBV was extracted from this cell line by treatment of the cells for 3 hr on ice with Tris buffer containing 10% glycerol. The treatment reduced the capacity of the cells to bind virus, and after concentration the receptor extract (RE) inhibited both EBV bind...

This study describes the presence of a receptor for fluid phase human C3 and C3b on Raji cell membranes. The binding of C3 and C3b was demonstrated indirectly by a fluoresceinated anti-C3 serum and directly by using radioiodinated proteins. No other complement proteins or serum factors were needed to mediate binding of C3 and C3b to the receptor. The possibility of enzymatic cleavage of C3 befo...

Abstract T-cell acute lymphoblastic leukemia (T-ALL) is a hardly curable disease with high relapse rate. 20 analogs were synthesized based on the structures of two kinds fungi-derived polyenylpyrrole products (rumbrin (1) and auxarconjugatin-B (2)) to suppress growth T-ALL-derived cell line CCRF-CEM tested for growth-inhibiting activity. The octatetraenylpyrrole analog gave an IC50 0.27 μM in c...

Curcumin has been documented to exert anticancer effects by interacting with altered proliferative and apoptotic pathways in cancer models. In this study, we evaluated the potential of curcumin to reverse promoter methylation of the p15 gene in Raji cells and its ability to induce apoptosis and genomic instability. Anti-neoplastic action of curcumin showed an augmentation in reactive oxygen spe...