Site-directed and random mutagenesis have been useful tools in molecular biology. The application of directed mutagenesis in medically important fungi has been limited by the availability of molecular genetic techniques. Even species in which efficient genetic transformation methodologies exist, mutagenesis approaches were sparsely used due to diploidism. Lack of genetic tools hindered understa...

Random mutagenesis has become a powerful means of studying the effects of a large number of permutations of a particular DNA sequence. As a prime example, libraries of randomized cDNA clones, when translated into their corresponding proteins, can be useful in investigating the functional contributions of a mutagenized region to the overall properties of a protein. Existing molecular cloning tec...

Random mutagenesis is a useful tool to genetically modify organisms for various purposes, such as adaptation to cultivation conditions, the induction of tolerances, or increased yield of valuable substances. This is especially attractive for systems where it is not obvious which genes require modifications. Random mutagenesis has been extensively used to modify crop plants, but even with the re...