The essential cell division protein FtsL is a substrate of the intramembrane protease RasP. Using heterologous coexpression experiments, we show here that the division protein DivIC stabilizes FtsL against RasP cleavage. Degradation seems to be initiated upon accessibility of a cytosolic substrate recognition motif.

Lipid modifications such as palmitoylation or myristoylation target intracellular proteins to cell membranes. Secreted ligands of the Hedgehog and Wnt families are also palmitoylated; this modification, which requires the related transmembrane acyltransferases Rasp and Porcupine, can enhance their secretion, transport, or activity. We show here that rasp is also essential for the developmental ...

In this paper, we extend our previous work where we have introduced the possibility of defining and using resources in ASP. In fact, in P-RASP (Resourced ASP with Preferences) one can define resources with their amounts. Available resources can be used for producing other resources, thus consuming either all or just a part of the available amount. The remaining amount, if any, can be used in a ...

Electrophysiological and video recording methods were used to investigate the function of lip touch in feeding ingestion behavior of the pond snail Lymnaea stagnalis. Although this stimulus was used successfully as a conditioning stimulus (CS) in appetitive learning experiments, the detailed role of lip touch as a component of the sensory stimulus provided by food in unconditioned feeding behav...

BACKGROUND The microbial cell factory Bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. Nonetheless, the effective secretion of particular heterologous enzymes remains challenging. Over the past decades various studies have tackled this problem, and major improvements were achieved by optimizing signal peptides or removing proteases invo...

Secure data intensive computing in the cloud is challenging, involving a complicated tradeoff among security, performance, extra costs, and cloud economics. Although fully homomorphic encryption is considered as the ultimate solution, it is still too expensive to be practical at the current stage. In contrast, methods that preserve special types of data utility, even with weaker security, might...

The access specification language RASP extends traditional role-based access control (RBAC) concepts to provide greater expressive power often required for fine-grained access control in sensitive information systems. Existing formal models of RBAC are not sufficient to describe these extensions. In this paper, we define a new model for RBAC which formalizes the RASP concepts of controlled role...

PURPOSE/OBJECTIVES The complex planning and quality assurance required for spine SBRT are a barrier to implementation in time-sensitive or limited resource clinical situations. We developed and validated an automated inverse planning algorithm designed to streamline planning and allow rapid delivery of conformal single fraction spine SBRT using widely available technology. MATERIALS/METHODS T...

In a software product family context, software architects anticipate product diversification and design architectures that support variants in both space (multiple contexts) and time (changing contexts). Product diversification is based on the concept of variability: a single architecture and a set of components support a family of products. Software product families need to support increasing ...

Now a day’s cloud is more popular because in cloud users host the data and upload a large contained data. It has large databases to database service providers so database service providers maintain the services of range query services. In clouding process some users have a sensitive private data in that situation user’s can’t move the data for hosting until we provide security, confidentiality,...