We have analyzed the repeated DNA fraction of the rat by characterizing approximately 500 repeat DNA-containing clones using hybridization to a variety of rodent nucleic acids. To facilitate this analysis we devised a method whereby the cloned DNA is transferred to nitrocellulose paper by blotting directly out of colonies of the bacterial clones. In addition to identifying repeated sequences of...

The bioactivation of the tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), by microsomes from target organs was studied with an in vitro microsome-mediated DNA alkylation system. Mouse lung, rat lung, and rat nasal microsomes catalyzed a time- and protein-dependent DNA methylation by [methyl-3H]NNK with activities of 4.11, 0.95, and 137.4 pmol/mg DNA/mg protein...

Background & Aims: Lipotoxicity is the process through which accumulation of lipid intermadiates leads to cellular dysfunction. The worldwide prevalence of cardiovascular diseases has been increased dramatically due to lipotoxicity. According to the peformed studies, saturated and unsaturated fatty acids differ significantly in their contributions to lipotoxicity. It has been r...

DNA repair is essential for maintaining the integrity of the genetic material, and a number of DNA repair mechanisms have been fairly well characterized for the nuclear DNA of eukaryotic cells as well as prokaryotes. However, little is known about DNA repair in mitochondria. Using highly sensitive immunoanalytical methods to detect specific DNA alkylation products, we found active removal of O6...

BACKGROUND AND AIMS The arterial wall calcium score and circulating free DNA levels are now used in clinical practice as biomarkers of cardiovascular risk. Calcium phosphate apatite retention in the arterial wall necessitates precipitation on an anionic platform. Here, we explore the role of tissue-free DNA as such a platform. METHODS The first step consisted of histological observation of sa...

The metabolism of several N-nitrosamines (N-nitrosodimethylamine, N-nitrosoethylmethylamine, N-nitrosodiethylamine, N-nitrosobenzylmethylamine, and N-nitrosopyrrolidine) in cultured human and rat esophagus has been investigated by measuring (a) CO2, (b) metabolites with an oxo group, and (c) metabolites bound to DNA. Both acyclic and cyclic N-nitrosamines were metabolized by rat esophagus. The ...

Ischemia/reperfusion is known to result in DNA fragmentation and cell death in kidney tubular epithelium, but the endonucleases responsible for this DNA damage have not been identified. DNA substrate gel analysis of extracts from normal rat kidney cortex revealed the presence of a DNase with an apparent molecular mass of 30 to 34 kD. This enzyme is not a dimer of the previously described nuclea...

DNA-binding non-histone proteins (NHP) from rat liver have been prepared by utilizing their affinity toward phosphocellulose. The specifity of the DNA binding sites was investigated by the reassociation kinetics of the protein-bound DNA. Isolated protein-bound DNA (350 nucleotides) reassociated with a a-fold increase in the content of middle repetitive sequences as found in the whole rat genome...

Background In tropical areas, Leptospirosis is still considered a public health problem, particularly in the event of heavy rainfall and flooding. Transmission leptospira bacteria from infected animals, such as rats, takes place through urine or blood. Different serovar leptospires can be found different reservoir animals field. However, research on presence pathogenic rats Pati Regency, one ar...

Previous reports that radioactivity was bound to rat liver DNA in vivo after administration of 2-acetylaminofluorene (AAF)-9-14C were confirmed. In addition, administration of several other AAF derivatives, namely N-hydroxy-AAF-9 -14C, N-acetoxy-AAF-914C, and the glucuronide of N-hydroxyAAF-9)4C, led to the binding of higher levels of radioactivity to rat liver ribosomal RNA and DNA. Maximum le...