Early, rapid effects of glucagon on glutamate metabolism were studied in rat livers perfused with bicarbonate buffer containing bovine albumin and red cells, [14C]glutamate in tracer amounts, and physiological levels of lactate and pyruvate. Glucagon increased [Wlglucose synthesis from [W]glutamate to a greater extent than from other labeled substrates. The effect was maximal at 3 min and was n...

New Zealand White rabbits were infused with [3H]tyrosine for periods of 5--6 h and then different methods of extraction were applied for the purification of the main muscle proteins and protein fractions. Myosin (I), prepared from salt extraction of muscle mince, consistently had a higher specific radioactivity than did myosin (II), isolated by dissociation of actomyosin. Actins (IA) and (IB), ...

The biosynthesis of fatty acids has been studied in lactating rabbits at 6h after intravenous injection of sodium [1-(14)C]acetate. The specific radioactivities of the individual fatty acids (C(6:0) to C(14:0)) and the proportions of these fatty acids synthesized were similar in mammary tissue and milk. Hexanoic acid had the highest specific radioactivity, and the C(8:0)-C(14:0) fatty acids had...

The reaction of a 100 Mo beam at 12 MeV/nucleon impinging on 4 He gas-cell target was performed. 99 alongside other coproduced isotopes were collected after the gas an aluminum catcher foil and their respective radioactivities measured by offline γ -ray analysis. In this contribution, preliminary experimental results which are used to discuss possibility optimal large-scale production condition...

The aim of this work was to determine 137Cs and 40K radioactivities in soil samples taken from the Babia Góra National Park (BPN) in south Poland. The cluster analysis (CA) and principal component analysis (PCA) were used to discuss the obtained data. 10 cm thick soil cores were collected from the BPN area. Each sample was divided into three sub-samples. The samples were dried, homogenized and ...

Euglena gracilis was grown in the dark for 12 days in a medium containing sodium acetate1-14C. The major amount of 14C was incorporated into the neutral lipids. In these compounds myristic acid was the main fatty acid labeled. Subsequent growth in light (70 hours) in an iden­ tical medium (containing unlabeled acetate) led to a strong decrease of radiocarbon in the neutral lipids. The radioacti...

objective: to assess relative biological effectiveness (rbe) of 131i radiation relative to 60co gamma rays in glioblastoma spheroid cells. materials and methods: in this experimental study, glioblastoma spheroid cells were exposed to 131i radiation and 60co gamma rays. radiation induced dna damage was evaluated by alkaline comet assay. samples of spheroid cells were treated by radiation from 13...