G RAEBER AND STUART’ reported an increase in procoagulant activity in the cerebrospinal fluid (CSF) of children with acute lymphoblastic leukemia (ALL) following prophylactic central ncrvous system (CNS) treatment with cranial radiation and intrathecal methotrexate. They postulated that the shortening of the plasma recalcification time (PRT) reflected the presence of brain phospholipid released...

Howell1 has shown that in testing blood to determine the presence of a hemophilic tendency, it is more satisfactory to obtain the clotting time of the oxalated and centrifugalized plasma after recalcification than to depend upon the time of coagulation of the whole blood, since in the latter case small variations in conditions may make large differences in the figures obtained. In connection wi...

A circulating anticoagulant which interferes with the formation of blood thromboplastin is well recognized as one cause of a haemorrhagic disorder. Several techniques for their demonstration have been described, depending on the ability of the patient's blood to prolong the clotting time of normal whole blood (Singer, Mond, Hyman, and Levy, 1950) or plasma (Biggs and Macfarlane, 1953), or to in...

The objective of this study was to identify and evaluate for the first time anticoagulant fibrinogenolytic protease from Psidium guajava seed extract. extract in sodium dodecyl sulfate polyacrylamide gel electrophoresis showed protein bands high molecular weight region between 122 kDa 47 two distinct lower at ~16.6 ~14.3 kDa. content found be 1 mg/ml with specific activity 0.8 U. proteolytic su...

The one abnormality constantly found in hemophilia is an inability of the blood to coagulate in a normal manner. This defect is demonstrable usually by a prolonged clotting time, which in turn determines the various manifestations of the disease. Further understanding of this disease depends, therefore, upon knowledge of the cause of the prolonged clotting time. Most investigations of the blood...

We have studied the effects of two human pancreatic cancer and two human small cell lung cancer cell lines on clotting and platelet aggregation. Both pancreatic lines markedly shortened recalcification times and induced platelet aggregation. The lung cancer lines produced little shortening of recalcification times and no platelet aggregation. The clotting and aggregation activities of the pancr...

Sequential alterations in the ultrastructure of blood platelets observed during the clotting of human platelet-rich plasma are described, with emphasis on disintegration of the platelet. As the clotting reaction proceeds, aliquots of citrated and recalcified citrated plasma are fixed by adding buffered OsO(4). After recalcification a lag period of about 10 minutes is followed by an interval of ...

In this study, anticoagulant activity was detected in salivary gland homogenates (SGHs) of Thyrsopelma guianense (Diptera: Simuliidae). The SGH yielded 1.07 microg +/- 0.03 (n = 15) of total soluble protein per pair of glands. In addition, following SDS-PAGE (12.5% gel) and silver nitrate staining, 12 polypeptides with molecular weights ranging from 14-69 kDa were detected in all physiological ...

In this study, three type II phenolic acids (caffeic acid, p-hydroxycinnamic acid, and ferulic acid) were used to synthesize a total of 18 phenolic acid derivatives. With molecular docking for molecule design and target protein (factors) screening, in combination with the confirmation of target proteins (factors) by surface plasmon resonance, and the evaluation of haemostatic and anticoagulant ...