RT-PCR amplification of P450 2C6 from rat liver, using primers in opposite orientations of exon 6, resulted in PCR products containing segments of exons joined at non-consensus splice sites. Moreover, many of the PCR products identified were composed of not only a single region containing exonic segments joined at non-consensus splice sites but, instead, of several repeats of the non-canonicall...

A series of comparative studies on the chemical and biological characterizations of the ovine lutropin subunits and their recombinants prepared by a countercurrent distribution (CCD) procedure and a salt precipitation method is presented. The salt precipitation Method B (0.1 M acetate and 3 M NaCl) gave a low yield of pure subunits and the subunits thus prepared recombined poorly to form a prod...

Part of the success a breeding program depends on composition base population. Superior genotypes and unimproved dioecious varieties are sources genes for traits interest excellent options formation segregating populations. Here we describe first cycle recurrent selection (RS) in papaya propose an effective strategy, unprecedented crop, to generate variability new cultivars. Initially, RS popul...

Tracing by means of the light rare earths (REs), particularly La and Ce, is a state-of-the-art method used to track deoxidation products during steelmaking process. Traced heterogeneous multiphase inclusions are analyzed using scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS) perform 2D characterization. The sequential chemical extraction technique implemented for 3D in...

This study examined whether amnesic patients and normal subjects can acquire novel associations implicitly and whether such learning can occur rapidly in a single trial. In two experiments, subjects studied novel word pairs either once or multiple times and were then asked to read old, new, and recombined word pairs as quickly as possible. In this paradigm, the learning of novel associations wo...

Abstract. This paper examines the effect of crossover operations on the performance of EMO algorithms through computational experiments on knapsack problems and flowshop scheduling problems using the NSGA-II algorithm. We focus on the relation between the performance of the NSGA-II algorithm and the similarity of recombined parent solutions. First we show the necessity of crossover operations t...