Box-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0-7.0), flow rate (1.0-1.4 mL/min), and mobile phase ratio (85-95% acetate buffer). Resolution values ...

Food colorants are important in food selection because they improve the gastronomic appeal of foods by improving their aesthetic appeal. However, after prolonged use, many turn toxic and cause medical problems. A synthetic azo-class dye called carmoisine gives meals a red color. Therefore, determination samples is great importance from human health control. The current work was developed to syn...

This work aims to evaluate the adsorption capacity of an abundant natural diatomite (ND) remove azo dye carmoisine, known as a harmful emerging organic pollutant. Indeed, best our knowledge, no results were reported on this subject. The ND was characterized by FTIR, XRD, and SEM/EDX analyses. experimental study carried out in batch mode. Results showed that adsorbent is mainly composed silica. ...

Abstract Overexposure to food colorants above the allowed daily intake (ADI) level can provoke hyperactivity and other disturbed behaviors especially in children. Two new methods were developed separate five synthetic colorants, which Tartrazine (E102), Sunset Yellow (E110), Allura Red (E129), Carmoisine (E122), Brilliant Blue (E133). They are labeled on a large variety of commercial products E...

Currently available techniques for performing quantitative immunohistochemistry (Q-IHC) rely upon pixel-counting algorithms and therefore cannot provide information as to the absolute amount of chromogen present. We describe a novel algorithm for true Q-IHC based on calculating the cumulative signal strength, or energy, of the digital file representing any portion of an image. This algorithm in...

This article describes the use of a power plant waste-Bottom Ash, as effective adsorbent for the removal of toxic Azo dyes Carmoisine and Chrysoidine Y from their aqueous solution. The paper incorporates comparative study and discussion of the adsorption characteristics of these dyes on the economically effective adsorbent with special emphasis on the effects of time and temperature. The charac...

The chemical pathway of the carbamidodiacetyl colorimetric assay was investigated. The experimental variables that were studied include reaction temperature, heating time, and the ratio of the mineral acid mixture to the reactants. Evidence is presented establishing the involvement of either 7or 8-methyl, or 7,8.d imethyltetrahyd roimidazo(4,5-d)imidazole-2,5-d iones as chromogens, all three be...

A simple, rapid and sensitive spectrophotometric method has been developed for the assay of ceterizine hydrochloride (CTZH) in bulk drug and its pharmaceutical preparations. This method is based on the ion-pair complex reaction between CTZH and Alizarin Red S in Clarks-Lubs buffer. The chromogen being extractable with chloroform, could be measured quantitatively at 440 nm. All variables were st...