نتایج جستجو برای: regulatable expression cassette
تعداد نتایج: 878522 فیلتر نتایج به سال:
The yeast three-hybrid system (Y3H), a powerful method for identifying RNA-binding proteins, still suffers from many false positives, due mostly to RNA-independent interactions. In this study, we attempted to efficiently identify false positives by introducing a tetracycline operator (tetO) motif into the RPR1 promoter of an RNA hybrid expression vector. We successfully developed a tight tetrac...
BACKGROUND Several plasmid DNA-based mammalian expression systems have recently been developed which make it possible to manipulate gene expression via the administration of exogenous agents. In order to extend the application of these systems, we have developed retroviral vectors which allow for the controlled expression of inserted genes both in vitro and in vivo. MATERIALS AND METHODS Two ...
Background: Parathyroid Hormone (PTH) is secreted by parathyroid glands and controls the level of calcium in bones and kidney. PTH is a small polypeptide with 84 amino acids, but the first 34 amino acids of which are enough for hormone biological activity and can be used in the treatment of Osteoporosis. The expression efficiency of recombinant human parathyroid hormone rhPTH (1-34) or Teripara...
background: interferons with diffrent functions such as antiviral, antiproliferative and immunomodulatory actions are effctivemedications for a number of diseases. one of these new interferons (ifns) is interleukin-29 (il-29) belongs to the family of ifn-λ hasantiviral activity and its potent in accompanying with ifn-α in treatment of hcv infection has been evaluated (clinical trial phase ii).r...
In view of recent serious adverse events and advances in gene therapy technologies, the use of regulatable expression systems is becoming recognized as indispensable adjuncts to successful clinical gene therapy. In the present work we optimized high-capacity adenoviral (HC-Ad) vectors encoding the novel tetracycline-dependent (TetOn)-regulatory elements for efficient and regulatable gene expres...
foot and mouth disease (fmd) is highly contagious disease among cloven-hoofed animals. fmd virus has structural and non-structural proteins. vp1 is the most immunogenic structural peptides of fmd virus, applied for major vaccine studies. objective: construction of pet28-vp1 cassette for fmd virus type o/irn/2010 and expression vp1 peptide as the most immunogenic antigen was the aim of this stud...
Lentiviral vectors (LVs) are considered one of the most promising vehicles to efficiently deliver genetic information for basic research and gene therapy approaches. Combining LVs with drug-inducible expression systems should allow tight control of transgene expression with minimal side effect on relevant target cells. A new doxycycline-regulated system based on the original TetR repressor was ...
background: uropathogenic escherichia coli (upec) are major bacterial agent of urinary tract infection (uti). this infection is more prevalent among women because approximately half of all women will experience a uti in their life-time and near a quarter of them will have a recurrent infection within 6–12 months. iuta protein has a major role during upec pathogenesis and consequently infection....
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