Most known plant disease-resistance genes (R genes) include in their encoded products domains such as a nucleotide-binding site (NBS) or leucine-rich repeats (LRRs). Sequences with unknown function, but encoding these conserved domains, have been defined as resistance gene analogues (RGAs). The conserved motifs within plant NBS domains make it possible to use degenerate primers and PCR to isola...

Article history: Received on: 12/03/2016 Revised on: 26/03/2016 Accepted on: 16/04/2016 Available online: 21/04/2016 Plants combat their pathogens with an array of defense responses. One of the key mechanisms involves products of resistance (R) genes which are responsible for recognition of effector molecules from pathogens and subsequent triggering of defense responses. Resistance gene analogu...

RGA and GAI are homologous genes that encode putative transcriptional regulators that repress gibberellin (GA) signaling in Arabidopsis. Previously we showed that the green fluorescent protein (GFP)-RGA fusion protein is localized to the nucleus in transgenic Arabidopsis, and expression of this fusion protein rescues the rga null mutation. The GA signal seems to derepress the GA response pathwa...

RGA (for repressor of ga1-3) and SPINDLY (SPY) are likely repressors of gibberellin (GA) signaling in Arabidopsis because the recessive rga and spy mutations partially suppressed the phenotype of the GA-deficient mutant ga1-3. We found that neither rga nor spy altered the GA levels in the wild-type or the ga1-3 background. However, expression of the GA biosynthetic gene GA4 was reduced 26% by t...

Polymer concrete (PC) is a composite construction material that boasts several advantages, such as lightness, low water permeability, high resistance to corrosive environments, and chemical degradation. Consequently, it has recently attracted interest an alternative the traditional ones for civil applications. In this study, unsaturated polyester resin was considered matrix phase of PC. Aimed p...

The identification, isolation and subsequent cloning of effective disease resistance genes for their use to enhance the protection level against various groups of pathogens in plants is an important aspect of crop improvement. Disease resistance genes (R-genes) have been characterized and cloned from many plant species. The resistance gene analogs (RGAs) are putative or tentative disease resist...

The phytohormone gibberellin (GA) is critical for anther development. RGA, a member of the DELLA family proteins that are central GA signalling repressors, key regulator male fertility in plants. However, downstream genes GA–RGA-mediated development remain to be characterised. We identified RGA Target 1 (RGAT1), novel Arabidopsis gene, functions as an important RGA-regulated target pollen RGAT1...

BACKGROUND Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton (Gossypium Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding. RESULTS Using the linker adaptor PCR (LA-PCR) with the primer...

A mutation in Regena (Rga) was isolated in screens for modifiers of white eye color gene expression. The reduction in the level of the Rga product results in a complex modulation of white mRNA both positively and negatively, depending on the developmental stage. In addition to white, Rga also affects the expression of several other tested genes, with one of them, Vinculin, being regulated in a ...