Numerous experimental techniques for the separation of transfer ribonucleic acids have been used successfully in preparing partially purified fractions of several specific tRNAs.1 Many of the existing methods have depended upon the differential solubility of specific tRNAs in complex two-phase systems (I-B), and the separations were achieved by means of countercurrent extraction techniques. Col...

A highly efficient technique has been developed for the resolution of several sterols that are intermediates in the biosynthesis of cholesterol and that differ only by one carbon-carbon double bond or by one methyl group. The technique described utilizes reverse-phase high-pressue liquid chromatography on a micronBondapak-C18 column with acetonitrile as eluting solvent. This procedure is capabl...

This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed....

A retention prediction model was developed for peptides separated in reversed-phase chromatography. The model was utilized to identify and exclude the false positive (FP) peptide identifications obtained via database search. The selected database included human proteins, as well as decoy sequences of random proteins. The FP peptide detection rate was defined either as number of retention time o...

Objective: The present investigation is aimed to develop and validate, a simple, consistent sensitive stability-indicating reverse phase-high performance liquid chromatography (RP-HPLC) method for the determination of oral anti-diabetic drug Canagliflozin in bulk pharmaceutical dosage form as per International Council Harmonization Technical Requirements Pharmaceuticals Human Use (ICH-Q2 (R1))....

styrene is an important constituent of widely used organic solvents in industries for production of various synthetic materials. the use of solid-phase extraction (spe) has grown and is a fertile technique of sample preparation as it provides better results than those produced by liquidliquid extraction. in this study, spe has been optimized, regarding sample ph, sample concentration, sample fl...

Reversed-phase chromatography is a method that is often used for glycan separation. For this, glycans are often derivatized with a hydrophobic tag to achieve retention on hydrophobic stationary phases. The separation and elution order of glycans in reversed-phase chromatography is highly dependent on the hydrophobicity of the tag and the contribution of the glycan itself to the retention. The c...