We describe the development and application of an expressed sequence tag (EST)-derived restriction fragment length polymorphism (RFLP) marker for sugarcane elite genotypes which can be used for quantitative trait loci (QTL) tagging for sugar content. EST-derived RFLP markers for proteins involved in sucrose metabolism have been used in Southern analysis for mapping and gene tagging in elite sug...

Molecular phylogeny and genetic diversity in all the nine species (Rhizophora mucronata, R. apiculata, R. stylosa, Bruguira cylindrical, B. parviflora, B. gymnorriza, Ceriops tagal and C. decandra) and a natural Rhizophora hybrid, of the mangrove Rhizophoraceae, occurring in the Indian sub-continent were analysed using random amplified polymorphic DNA (RAPD), restriction fragment length polymor...

background: indirect genetic diagnosis using polymorphic dna markers can detect carriers of hemophilia a. this technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. in the present study, we examined usefulness of intragenic marker bcl i restriction fragment length polymorphism (rflp) at intron 18, for carrier detec...

The genus Bacillus comprises of a diverse group with a wide range of nutritional requirements and physiological and metabolic diversity. Their role in nutrient cycle is well documented. 16S rDNA sequences do not always allow the species to be discriminated. In this study 40 Bacillus spp. obtained from fish culture pond and 10 culture type strains were analysed for their genomic diversity by PCR...

In Iraq generally and Kurdistan region specifically, goats are an important resource for meat milk production. It is well known that Myostatin genes (MSTN) have the essential role in growth development due to its crucial involvement muscle growth. The goal of this research was reveal polymorphism MSTN gene different Capra hircus breeds (native, Shami, Meriz Kamori goat) wild mountain goat (Capr...

highly informative DNA markers evenly spaced throughout the genome. A number of molecular genetic maps of the soybean [Glycine max Using a mapping population derived from an inter(L.) Merr.] have been developed over the past 10 yr. These maps are specific G. max 3 G. soja cross, Shoemaker and Olson primarily based on restriction fragment length polymorphism (RFLP) (1993) developed a molecular g...

The integration of genetic and physical maps of maize is progressing rapidly, but the cytogenetic maps lag behind, with the exception of the pachytene fluorescence in situ hybridization (FISH) maps of maize chromosome 9. We sought to produce integrated FISH maps of other maize chromosomes using Core Bin Marker loci. Because these 1 Kb restriction fragment length polymorphism (RFLP) probes are b...

BACKGROUND Chronic lung infections are the primary cause of morbidity and mortality in Cystic Fibrosis (CF) patients. Recent molecular biological based studies have identified a surprisingly wide range of hitherto unreported bacterial species in the lungs of CF patients. The aim of this study was to determine whether the species present were active and, as such, worthy of further investigation ...

In this study, a PCR-RFLP method was employed to investigate the evidence of two mutation sites in exon 2 (c.N8S) and 3 (c.E36Q) regions AQP7/TaaI gene Madura bulls. Both were polymorphic with presence three genotypes. However, c.N8S had moderate PIC value (>0.30). Hence, it can be suggested that used for genetic marker sperm quality traits association study 15 selected bulls revealed there ...