Of 19 Rhizopus spp. only four produced L-lactic acid in shake-flask culture. Aerobically and in the presence of a neutralizing agent, Rhizopus oryzae NRRL 395 produced the highest concentration of L-lactic acid (65 g/l) but with O2-limited growth ethanol was produced instead.

Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae gl...

Posaconazole and itraconazole were more potent inhibitors of ergosterol synthesis, in both intact cells and cell extracts from Absidia corymbifera and Rhizopus oryzae, than voriconazole and fluconazole. Similarly, expression of CYP51 from R. oryzae in Saccharomyces cerevisiae significantly increased resistance to fluconazole and voriconazole but not to posaconazole and itraconazole.

Rhizopus oryzae is reported for the first time on Cucumis melo L. var. makuwa Makino. A detailed description of this Korean specimen is given, along with its rDNA internal transcribed spacer sequence. On the basis of mycological characteristics and molecular data, the fungus was identified as R. oryzae Went & Prinsen Geerligs.

Rhizopus oryzae is an important organism for its production of organic acids such as lactic acid, fumaric acid, etc. To date, there were no easy methods to classify strains according to their acid production. The sequences of the ribosomal RNA-encoding DNA (rDNA) internal transcribed spacer (ITS) region of 64 strains of R. oryzae were analyzed and found to conserve mutations correspond to acid ...

Mika Murabayashi1, Taku Sugita1, Keiko Hirakawa2,3, Hideto Kuribayashi4, Ikuo Mori1, Kazuki Kubo1, Takashi Horiguchi1, Masayuki Ueda2,5, Yasuo Katayama2,5 and Youkichi Ohno2,3 Takeda Pharmaceutical Company Limited NMR and Small Animal MRI Laboratory, Nippon Medical School Department of Legal Medicine, Nippon Medical School Varian Technologies Japan Ltd. Division of Neurology, Department of Inte...

The production cost of β-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (SSF). In the present study, the effect of different production conditions on the yield of β-glucosidase and endoglucanase by Rhizopus oryzae MTCC 9642 from water hyacinth was in...

Polythene is considered as one of the important object used in daily life. Being versatile in nature and resistant to microbial attack, they effectively cause environmental pollution. In the present study, biodegradation of low-density polyethylene (LDPE) have been performed using fungal lab isolate Rhizopus oryzae NS5. Lab isolate fungal strain capable of adhering to LDPE surface was used for ...

Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated tha...

A Potential Explanation of a Positive Serum β-Glucan Assay in Mucormycosis To the Editor—I read with interest the nice study by Angebault et al [1] that gives a " real world " picture of the subop-timal performance of the serum β-glucan assay. Unexpectedly, the authors found that serum β-glucan test was positive in 3 of the 6 cases of mucormycosis in their series with no apparent cause of false...