نتایج جستجو برای: rna sequencing
تعداد نتایج: 355158 فیلتر نتایج به سال:
Background: Stenotrophomonas maltophilia (SMA) has emerged as an important pathogen capable of causing opportunistic and nosocomial infection. We performed RNA sequencing (RNA-seq) lung tissues from mice with pulmonary SMA infection over time via aerosolized intratracheal inhalation to investigate transcription profile changes in SMA-infected lungs.
Abstract DDX39B is a member of the DEAD box (DDX) RNA helicase family required for nearly all cellular metabolic processes. The exact role and potential molecular mechanism in progression human colorectal cancer (CRC) remain to be investigated. In present study, we demonstrate that expression higher CRC tissues than adjacent normal tissues. Gain- loss-of-function assays revealed facilitates met...
RNA sequencing is the use of high throughput next generation sequencing technology to survey, characterize, and quantify the transcriptome of a genome. RNA sequencing has been used to analyze the pathogenesis of several malignancies such melanoma, lung cancer, and colorectal cancer. RNA sequencing can identify differential expression of genes (DEG's), mutated genes, fusion genes, and gene isofo...
RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows mu...
Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify the quality of RNA, there have been no large-scale empirical evaluations of the major determinants of sequencing success. We used a combination of existing and newly developed methods to isolate total RNA from 1115 samples from 695 pla...
A recently developed technique called RNA Sequencing (RNA-Seq) uses massively parallel sequencing to allow transcriptome analyses of genomes at a far higher resolution than is available with Sanger sequencing- and microarray-based methods. In the RNA-Seq method, complementary DNAs (cDNAs) generated from the RNA of interest are directly sequenced using next-generation sequencing technologies. Th...
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