Overproduction of genetically engineered antibodies, such as single-chain antibodies (scAbs) in Escherichia coli often results in insoluble and inactive products known as inclusion bodies. We now report that fusion or co-expression of FkpA, the E. coli periplasmic peptidyl-prolyl-isomerase with chaperone activity, substantially improves soluble and functional expression of scAbs. Anti-human bla...

An outbreak of goatpox was attended in district Durg and surrounding regions of Chhattisgarh state. Dried skin scabs were collected from 250 goats of different age groups showing clinical signs suggestive of pox. The prevalence rate was studied based on severity of clinical signs followed by confirmation with agar gel immuno diffusion (AGID) test. Positive scabs were further processed for virus...

BACKGROUND We aimed to combine the generation of "artificial" antibodies with a rat pituitary bioassay as a new strategy to overcome 20 years of difficulties in the purification of gonadotrophin surge-attenuating factor (GnSAF). METHODS A synthetic single-chain antibody (Tomlinson J) phage display library was bio-panned with partially purified GnSAF produced by cultured human granulosa/luteal...

This paper presents an automated inspection algorithm for a thick plate. Thick plates typically have various types of surface defects, such as scabs, scratches, and roller marks. These defects have individual characteristics including brightness and shape. Therefore, it is not simple to detect all the defects. In order to solve these problems and to detect defects more effectively, we propose a...

Cutaneous protothecosis is a rare infection caused by achlorophyllic algae of the genus Prototheca. The lesions usually occur on exposed areas, related with trauma, in immunocompromised patients. The most common clinical presentation is a vesicobullous and ulcerative lesion with pustules and scabs, simulating bacterial, fungal or herpetic infections or eczema. The diagnosis is determined by age...

rarity of the affection in these parts. Nazir Mirza Mahommedan male, Sliiah ; age about 55 years ; resident of Lucknow city. He has been all his life-time in Lucknow, going once only on a pilgrimage to Bagdad about a year and a half ago. History,?The patient stated that he always enjoyed perfect health until about three months ago, when lie noticed a few itching papules, "like mosquitoe bites" ...

BACKGROUND AND OBJECTIVES Epistaxis is one of the common symptoms encountered in the Otorhinolaryngology department. Many times the cause for epistaxis is not found on anterior and posterior rhinoscopy. The present study was undertaken to assess the role of rigid nasal endoscope in the diagnosis and treatment of epistaxis, where normal anterior and posterior rhinoscopy did not reveal any specif...

Clinical and Epidemiologic Characteristics of Smallpox Smallpox is a viral disease unique to humans. To sustain itself, the virus must pass from person to person in a continuing chain of infection and is spread by inhalation of air droplets or aerosols. Twelve to 14 days after infection, the patient typically becomes febrile and has severe aching pains and prostration. Some 2 to 3 days later, a...