The influence of limited proteolysis of soy protein concentrate on protein extractability, the composition of the extractable proteins, their emulsifying properties and some nutritional properties were investigated. Traditional concentrate (alcohol leached concentrate) was hydrolyzed using trypsin and pepsin as hydrolytic agents. Significant differences in extractable protein composition betwee...

Development of multi drug resistant organism has been high due to improper use of antibiotics. That made the necessity to develop new drug molecules. In this study an effort was made to find a new alternative. A wild type microorganism was isolated from soil and was identified as Bacillus and confirmed as Bacillus subtilis species by 16S r RNA sequencing. The strain was identified to have the a...

A fatty acid hydroperoxidelyase (HPL) enzyme from soybean (Glycine max) seeds was purified by ammonium sulphate precipitation followed by ion exchange chromatography on DEAE-Cellulose and DEAE-Sephacel. HPL showed specific activity of 161U/mg with fold purification of 16.4 and enzyme yield was found to be 9.06%. The purified enzyme on SDSPAGE showed a prominent band of ~53KDa. Kinetic studies w...

Fig 6. Effect of Zn -enrichment on claudin-1 displacement rate. (A) NP-40-soluble and (B) NP-40insoluble fractions. Gray: calcium-free medium, black: Zn-enriched medium, white: Zn-enriched medium treated with STb for 6, 12 and 24 h. Lower panel: Immunoblot showing claudin-1 and GAPDH used to evaluate their relative amounts. NP-40 cell extracted proteins were separated on a 12% acrylamide SDSPAG...

A highly efficient cellobiohydrolase (CBH)-secreting basidiomycetous fungus, Agaricus arvensis KMJ623, was isolated and identified based on its morphological features and sequence analysis of internal transcribed spacer rDNA. An extracellular CBH was purified to homogeneity from A. arvencis culture supernatant using sequential chromatography. The relative molecular mass of A. arvencis CBH was d...

Two bacterial species; Pseudomonas fluorescens and Pseudomonas putida and three different chemical compounds; copper sulphate (CuSO4), Indole butyric acid (IBA) and potassium chloride (KCl) were tested for their ability to induce resistance in lupine plants against wilt disease caused by Fusarium oxysporum f. sp. lupini. Treatment of seeds with the selected bacterial species and chemical compou...

An anticoagulant protein was purified from the edible portion of a blood ark shell, Scapharca broughtonii, by ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50, Sephadex G- 75, DEAE-Sephacel, and Biogel P-100. In vitro assays with human plasma, the anticoagulant from S. broughtonii, prolonged the activated partial thromboplastin time (APTT) and inhibited the factor ...

The monoclonal antibody (MAb-001), which was produced against a surface membrane glycoprotein on C. salmositica, significantly inhibited the activities of the intracellular proteases of the parasite. The total activity in the partially purified metallo-protease, and about 80% of activity in the partially purified cysteine protease, were inhibited by the antibody (at 10 μg protein ml–1). The inh...

Several sulphated polysaccharides have been shown to block the infection of T-cells by the human immunodeficiency virus (HIV)-l [1,2]. Dextrin-2-sulphate (D2S) is a synthetically sulphated polysaccharide which inhibits infection of human T-cell lines by a variety of HIV-1 cell-free isolates [3]. Although its mechanism of action has not been established, this compound appears to prevent HIV-1 in...

In the horse, drug metabolism has been shown to be complex, but little is known about the proteins involved in these processes [l]. As a result of work on the purification of equine Cytochrome P450 enzymes, an epoxide hydrolase has been purified [2]. Epoxide hydrolases catalyse the hydration of biologically active epoxides formed during the metabolism of many drugs and mutagenic compounds [3]. ...