نتایج جستجو برای: secretion efficiency

تعداد نتایج: 516673  

2015
Ping Wang Lu Huang Hu Jiang Jian Tian Xiaoyu Chu Ningfeng Wu

BACKGROUND Although Pichia pastoris has been successfully used to produce various recombinant heterologous proteins, the efficiency varies. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as an example to study the effect of protein amino acid sequence on secretion from P. pastoris. RESULTS The results indicated that the protein N-terminal sequence, the endo...

Journal: :BMC Biotechnology 2005
Andreas Schaaf Stefanie Tintelnot Armin Baur Ralf Reski Gilbert Gorr Eva L Decker

BACKGROUND Efficient targeting to appropriate cell organelles is one of the bottlenecks for the production of recombinant proteins in plant systems. A common practice is to use the native secretory signal peptide of the heterologous protein to be produced. Though general features of secretion signals are conserved between plants and animals, the broad sequence variability among signal peptides ...

2011
Roger Geiger Matthias Gautschi Friederike Thor Arnold Hayer Ari Helenius

Although bovine pancreatic RNase is one of the best characterized proteins in respect to structure and in vitro refolding, little is known about its synthesis and maturation in the endoplasmic reticulum (ER) of live cells. We expressed the RNase in live cells and analyzed its folding, quality control, and secretion using pulse-chase analysis and other cell biological techniques. In contrast to ...

Journal: :Protein engineering, design & selection : PEDS 2012
A Gorlani D Lutje Hulsik H Adams G Vriend P Hermans T Verrips

Variable domains of llama heavy-chain antibodies (VHH) are becoming a potent tool for a wide range of biotechnological and medical applications. Because of structural features typical of their single-domain nature, they are relatively easy to produce in lower eukaryotes, but it is not uncommon that some molecules have poor secretion efficiency. We therefore set out to study the production yield...

Journal: :Japanese Journal of Infection Prevention and Control 2017

1999
BO AHRÉN CHRISTIAN THOMSEN

Ahrén, Bo, Per Sauerberg, and Christian Thomsen. Increased insulin secretion and normalization of glucose tolerance by cholinergic agonism in high fat-fed mice. Am. J. Physiol. 277 (Endocrinol. Metab. 40): E93–E102, 1999.— Increased insulinotropic activity by the cholinergic agonist carbachol exists in insulin-resistant high fat-fed C57BL/6J mice. We examined the efficiency and potency of carba...

Journal: :international journal of environmental research 0
n. amirrajab department of medical laboratory sciences, school of paramedicine/ infectious & tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, iran i. haghani department of medical mycology and parasitology/invasive fungi research center (ifrc), school of medicine, mazandaran university of medical sciences, sari, iran m. rasuli student research committee, school of medicine, mazandaran university of medical sciences, sari, iran t. shokohi department of medical mycology and parasitology/invasive fungi research center (ifrc), school of medicine, mazandaran university of medical sciences, sari, iran

migratory birds can become long-distance vectors for a wide range of microorganisms. the objective of the present study was to investigate the presence of cryptococcus neoformans in the cloacae, crop and nasal cavity of migratory birds in order to assess their role as potential reservoirs and/or mechanical vectors of human and animal cryptococcosis. a total of 700 samples (cloacae, crops and na...

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