BACKGROUND Although Pichia pastoris has been successfully used to produce various recombinant heterologous proteins, the efficiency varies. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as an example to study the effect of protein amino acid sequence on secretion from P. pastoris. RESULTS The results indicated that the protein N-terminal sequence, the endo...

BACKGROUND Efficient targeting to appropriate cell organelles is one of the bottlenecks for the production of recombinant proteins in plant systems. A common practice is to use the native secretory signal peptide of the heterologous protein to be produced. Though general features of secretion signals are conserved between plants and animals, the broad sequence variability among signal peptides ...

Although bovine pancreatic RNase is one of the best characterized proteins in respect to structure and in vitro refolding, little is known about its synthesis and maturation in the endoplasmic reticulum (ER) of live cells. We expressed the RNase in live cells and analyzed its folding, quality control, and secretion using pulse-chase analysis and other cell biological techniques. In contrast to ...

Variable domains of llama heavy-chain antibodies (VHH) are becoming a potent tool for a wide range of biotechnological and medical applications. Because of structural features typical of their single-domain nature, they are relatively easy to produce in lower eukaryotes, but it is not uncommon that some molecules have poor secretion efficiency. We therefore set out to study the production yield...

Ahrén, Bo, Per Sauerberg, and Christian Thomsen. Increased insulin secretion and normalization of glucose tolerance by cholinergic agonism in high fat-fed mice. Am. J. Physiol. 277 (Endocrinol. Metab. 40): E93–E102, 1999.— Increased insulinotropic activity by the cholinergic agonist carbachol exists in insulin-resistant high fat-fed C57BL/6J mice. We examined the efficiency and potency of carba...

migratory birds can become long-distance vectors for a wide range of microorganisms. the objective of the present study was to investigate the presence of cryptococcus neoformans in the cloacae, crop and nasal cavity of migratory birds in order to assess their role as potential reservoirs and/or mechanical vectors of human and animal cryptococcosis. a total of 700 samples (cloacae, crops and na...