Background: Hypothalamus-derived kisspeptins are critical regulators of reproduction in nearly all mammalian species including the humans. These small peptides mediate their actions through the GnRH loop system. How kisspeptins regulate gonadal maturation in sexually immature male mammals remains elusive. To address this, kisspeptin was administered as subchronic (12 days) twice daily i. p. dos...

In this study, the vegetative anatomy of Artemisia umbelliformis subsp. eriantha, a Central European alpine glacial relict, wild-growing on Mt. Durmitor (Montenegro) was examined for first time. The aim to investigate general and particular anatomical traits which might have possible taxonomic value. Microscopic slides were prepared according standard histological procedures. adventitious young...

The pancreatic acinar carcinoma established in rat by Reddy and Rao (1977, Science 198:78-80) demonstrates heterogeneity of cytodifferentiation ranging from cells containing abundant well-developed secretory granules to those with virtually none. We examined the synthesis intracellular transport and storage of secretory proteins in secretory granule-enriched (GEF) and secretory granule-deficien...

Neuroendocrine cells are specialized to produce, maintain and release large stores of secretory peptides. We show that the Drosophila dimmed/Mist1 bHLH gene confers such a pro-secretory phenotype on neuroendocrine cells. dimmed is expressed selectively in central and peripheral neuroendocrine cells. In dimmed mutants, these cells survive, and adopt normal cell fates and morphology. However, the...

The first phase of insulin secretion is altered during the early stages diabetes and followed by progressive loss ability glucose to stimulate secretion. primary causes secretory defects are still unknown. Better understanding signaling pathways linked activation G-protein–coupled receptors their mutual interactions in β-cells remains crucial. This represents a prerequisite for any strategy dev...

Introduction The expression of genes in female reproductive organs is influenced by the cyclic changes hormone levels during menstrual cycle. While molecular endometrium that facilitate embryo implantation have been extensively studied, there limited knowledge about impact cycle on cervical cells. Cervical cells can be easily and routinely collected using a cytobrush gynecological examination, ...

Background: The expression of bio-therapeutic proteins in mammalian cells, such as CHO, attains high homogeneity related to post-translational modifications. Although CHO remains the most popular cell line for bestselling biotherapeutic proteins on the market, there are still drawbacks such as expensive culture media, long time line, and high drug cost. Recently, researches on a novel Leishmani...