Secretory vesicles are neutrophil intracellular storage granules formed by endocytosis. Understanding the functional consequences of secretory vesicle exocytosis requires knowledge of their membrane proteins. The current study was designed to use proteomic technologies to develop a more complete catalog of secretory vesicle membrane proteins and to compare the proteomes of secretory vesicle and...

bacterial utilize a multitude of methods to secretion of specific substrate across phospholipid membranes. these bacterial strategies can play many roles in promoting bacterial process, from enhancing pathogenesis to bacterial response to environmental condition. the secretion in bacterial species transfer and release performance substance such as proteins, enzymes and metabolites to environmen...

Pichiapastoris is one of the most widely used expression systems for the production of recombinant secretory proteins. Its universal application is, however, somewhat hampered by its unpredictable yields for different heterologous proteins, which is now believed to be caused in part by their varied efficiencies to traffic through the host secretion machinery. The yeast endoprotease Kex2 removes...

Intercellular communication is fundamental in many biological processes involved in cell growth, differentiation, development, and reproduction of living organisms and secretory proteins are among the most important messengers in this network of information. The vast majority of extracellular proteins are exported from cells by the endoplasmic reticulum/Golgi-dependent secretory pathway. Howeve...

background: the aim of this study was to evaluate the protein spots of excre-tory - secretory products of fasciola hepatica using two dimension electrophoresis method in the presence and absence of triclabendazole drug which can be consid-ered to detect the target protein of the drug. methods: f. hepatica parasites were collected from infected cattle livers, divided in two groups and cultivated...

Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

background : rhoptries are unique secretory/excretory organelles that are found exclusively in the apicomplexa, and their contents are discharged at the time of invasion and are critical in the establishment of productive infection. several rhoptry proteins have been identified in toxoplasma gondii , plasmodium falciparum and neospora caninum and have been linked not only with the parasites’ ad...

Exocrine cells have an essential function of sorting secreted proteins into the correct secretory pathway. A clear understanding of sorting in salivary glands would contribute to the correct targeting of therapeutic transgenes. The present work investigated whether there is a change in the relative proportions of basic proline-rich protein (PRP) and acidic PRPs in secretory granules in response...