Here we present an inexpensive method to fabricate microscopic cellular cultures, which does not require any surface modification of the substrate prior to cell seeding. The method utilizes a reusable elastomeric stencil (i.e., a membrane containing thru holes) which seals spontaneously against the surface. The stencil is applied to the cell-culture substrate before seeding. During seeding, the...

Light is an important resource that crops and weeds compete for and so increased light interception by the crop can be used as a method of weed suppression in cereal crops. This research investigated the impact of altered availability of photosynthetically active radiation (PAR) (from crop row orientation or seeding rate) on the growth and fecundity of Lolium rigidum. Wheat and barley crops wer...

Seeding strategies for influence maximization in social networks have been studied for more than a decade. They have mainly relied on the activation of all resources (seeds) simultaneously in the beginning; yet, it has been shown that sequential seeding strategies are commonly better. This research focuses on studying sequential seeding with buffering, which is an extension to basic sequential ...

Seeding rangeland following wildfire is a central tool managers use to stabilize soils and inhibit the spread of invasive plants. Rates of successful seeding on arid rangeland, however, are low. The objective of this study was to determine the degree to which water availability, invasive plant abundance, and seeding technology influence postfire seedling establishment. Across four fire complexe...

In this paper, we investigate the ability to drive fluid streaming via a surface acoustic wave (SAW) into a porous bioscaffold structure, and to exploit this effect to deliver fluorescent particles/yeast cells into the scaffold as a potential rapid and efficient method for cell seeding in tissue engineering. The results demonstrate that the seeding process takes approximately 10 seconds, much s...

BACKGROUND The basic strategy to construct tissue engineered bone graft (TEBG) is to combine osteoblastic cells with three dimensional (3D) scaffold. Based on this strategy, we proposed the "Totally Vitalized TEBG" (TV-TEBG) which was characterized by abundant and homogenously distributed cells with enhanced cell proliferation and differentiation and further investigated its biological performa...

The flow field has significantly impact on seeding performance in the suction seeding device. A three-dimensional, incompressible, viscous, RNG turbulence model and the SIMPLE method were used by computational fluid dynamics(CFD), and the flow fields of suction cylinder-seeder with different socket’s radiuses were simulated by Fluent. When vacuum is 4kPa and productivity is 350 trays/h, the sim...

Protein crystals obtained in initial screens typically require optimization before they are of X-ray diffraction quality. Seeding is one such optimization method. In classical seeding experiments, the seed crystals are put into new, albeit similar, conditions. The past decade has seen the emergence of an alternative seeding strategy: microseed matrix screening (MMS). In this strategy, the seed ...

Surface acoustic waves (SAW) have been employed to drive a particle suspension into a porous scaffold as a means for cell seeding. Straight, simple interdigital electrode structures were fabricated on lithium niobate to permit the generation of Rayleigh SAW radiation. Fluorescent microscopy was used to investigate the seeding process; the SAW-driven seeding process occurred in approximately 10s...