The aim of this study was to define a simple extender for freezing rabbit semen from selected males used to inseminate selected doses to obtain embryos for an embryo bank. Four experiments were carried out. In the first experiment, freezing extender was defined on the basis of the results of the post-thawing motility rate. Three factors and their interactions were studied: final concentration o...

Egg yolk low-density lipoproteins (LDL) and soybean lecithin were evaluated as replacements for egg yolk in extenders used for the cryopreservation of brown-bear spermatozoa. The motility, viability and acrosomal status of post-thawed spermatozoa were analysed, and an egg-yolk extender was used as a control. The total antioxidant capacity of these extenders was tested. Soybean lecithin showed a...

The objective of the study was to assess the semen characteristics of Jersey, Mithun and Siri breeds of bulls and to compare the suitability of Tris Eggyolk Citrate and commercial soya lecithin based BIOXcell extenders for cryopreservation of semen from the these breeds of bulls. The mean semen volume was 4.55± 1.57, 4.86±1.33 and 4.83±1.72 ml in Jersey, Mithun and Siri bulls, respectively. Mea...

The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected ejaculates, only semen samples with motility of more than 70% and sperm concentration higher than 3×10 sperm/ml were used for cryopreservation. The selected semen samples were then pooled and dilu...

the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and...

The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolk-based extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined a...

Background: Semen cryopreservation is the most important section of artificial insemination programs; it allows preservation of semen fertility for a long time. Materials and Methods: The aim of the present study was to detect the effect of in vitro supplementation of 4-hydroxy Tempo on fresh and frozen spermatozoa quality of buffalo bulls. Five healthy buffalo bulls (5 ejaculates from each bul...

Caffeine was incorporated in egg yolk tris glycerol extender (EYTG) at the time of dilution of Murrah semen at a concentration of 1.0, 3.0 or 5.0 mM. The samples were subsequently frozen. The post thaw progressive motility and live sperm per cent improved signifi cantly (P<0.05) with addition of 1.0 (70.0±0.99 and 88.80±0.84) and 3.0 mΜ (65.83±0.88 and 86.16±0.96%) caffeine to the extender befo...

the aim of the present research was to study the effect of different concentrations of rosemary (rosmarinus officinalis l.) extract, (0, 10, 12.5, 16.6, 25 and 50) mg/l added to semen extender, on sperm qualitative and quantitative parameters after freezing-thawing process of rooster sperm. 10 ross strain rooster were used. semen samples were collected, 2 times a week. after adding the samples ...

In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucro...