Highly purified human plasminogen has been prepared from a variety of plasma fractions and by different purification methods. The two major fractions used have been serum or plasma euglobulin and Cohn plasma Fraction III. The purification methods (1-14) have all yielded products of high purity. Plasminogen useful for further purification and isolation studies can be prepared by the Kline method...

Knowledge of the reserve bilirubin-binding capacity is useful in management of hyperbilirubinemia in the newborn. We describe thin-layer gel chromatography that permits measurement of this variable, with a 250-mul sample of serum, within 50 min. Single samples from four different infants can be analyzed on the same plate. The capacity of 40 sera from jaundiced and of 19 sera from nonjaundiced i...

griseoaurantiacus SANK63479. In this paper, we report the isolation procedure and structural elucidation of griseolic acids B2) and C3). Griseolic acids B and C were separated from the mother liquid of griseolic acid A by Sephadex G-10 column chromatography. The mother liquid was dissolved in water and applied on Sephadex G-10 column and developed with water. Two active fractions (griseolic aci...

Lipoxygenase (LOX) is an enzyme that catalyses the step-wise oxygenation of polyunsaturated fatty acids (PUFAs) to form acid hydroperoxides within living tissues. In present study, lipoxygenase was isolated from defatted and whole (full fat) conophor nut (Tetracarpidium conophorum) purified homogeneity using 60% ammonium sulphate precipitation, DEAE-sephadex A50 sephadex G-200 chromatography. T...

Flavonoid compound was isolated from Crinum amabile Donn. leaves. The separation of flavonoids carried out by using chromatographic techniques to purify CH2Cl2 extract Column chromatography with silica gel and sephadex as stationary phases, thin-layer chromatography, preparative were the used in this research. pure obtained form white-yellow solid Rf value is 0.44. Compound identification 1H-NM...

With the aid of more sensitive instrumental methods it is possible to determine trace elements after separation of different fractions from soil and plant samples. Fractions of soils and sediments are obtained by extraction with different reagents, chosen in function of biological, ecological or chemical considerations. In plant samples it is possible to separate free ions and organo-mineral co...

The folic acid activity (FAA) of the protein zone in DEAE Sephadex A-50 chromatography of normal serum showed the following distribution: most (about 40%) was eluted with protein zone IV (alpha-2-macroglobulin), about 33% with protein zones II-III (transferrin), and the balance with zone V (albumin). In healthy human subjects the variations were relatively small. The repeatability of the chroma...