We typed 100 unrelated, healthy Poles from the region of Lower Silesia for HLA-C using low-resolution polymerase chain reaction sequence-specific primers (PCR-SSP) and compared the observed allele frequencies with data published for other human populations. Poles appeared to be most similar to the Germans and English more distant from the French, Catalans and Basques and most dissimilar to non-...

BACKGROUND Narcolepsy is a neurologic disorder characterized by excessive daytime sleepiness, symptoms of abnormal rapid eye movement (REM) sleep, and a strong association with HLA-DRB1*1501, -DQA1*0102, and -DQB1*0602. Here, we investigated the clinico-physical characteristics of Korean patients with narcolepsy, their HLA types, and the clinical utility of high-resolution PCR with sequence-spe...

Objective: This study aimed to investigate the association between HLA alleles and visceral leishmaniasis (VL) in a sample of Iraqi patients. Methods: A total of 30 patients were studied, in addition to 20 age, gender and ethnicity matched controls. All subjects were genotyped by polymerase chain reaction-sequence specific primers (PCR-SSP) method. Results: For HLA-class I region (A and B loci)...

Objective: This study aimed to investigate the association between HLA alleles and visceral leishmaniasis (VL) in a sample of Iraqi patients. Methods: A total of 30 patients were studied, in addition to 20 age, gender and ethnicity matched controls. All subjects were genotyped by polymerase chain reaction-sequence specific primers (PCR-SSP) method. Results: For HLA-class I region (A and B loci)...

the association of hla class ii genes with ulcerative colitis (uc) as an autoimmune disease has been investigated for several years. however, factors responsible for genetic predisposition of this disease have so far not been clearly understood. in this study, for the first time, we aimed to investigate the association between hla-drb1 types and uc in the population of kerman, a city southeast ...

BACKGROUND DNA-based methods are useful for enhancing immunohematology typings. Ready-to-use Conformité Européenne (CE)-marked test kits based on polymerase chain reaction with sequence-specific priming (PCR-SSP) have been developed, which enable the examination of weak, unexpected, or unclear serologic findings. DEVELOPMENT AND VALIDATION: Primers were designed according to established mutatio...

BACKGROUND AND OBJECTIVES Although widely used, serological typing of HLA loci does not always produce uequivocal results. This may be particularly the case for cord blood since samples may be of small volume and poor quality, and contaminated. DESIGN AND METHODS We typed 220 cord blood units (CBU) for HLA class I antigens using the serological technique. For those samples giving doubtful res...

DNA based HLA typing is fast replacing conventional Microlymphocytotoxicity based method, which has been regarded as the gold standard. Many laboratories in Mumbai have already switched over to molecular methods, as the results are far superior. This study was undertaken to compare the results of generic molecular typing by sequence specific primers (SSP) with that by serology in our laboratory...

Thesis Abstract Several cytokines are known to be involved in the pathogenesis of non-Hodgkin Lymphomas (NHL). Given importance understanding genetic predisposition NHL, this work was designed study impact IL-10 (-1082 G/A; rs1800896 and -819 C/T; rs1800871), IL-6 (-174 G/C; rs1800795) CD38 (184C/G; rs6449182) gene polymorphisms on susceptibility Egyptians NHL. Both loci IL10 were genotyped usi...

systemic lupus erythematosus (sle) is an autoimmune disease in which polymorphisms within the human leukocyte antigen (hla) region have been associated to its etiology. we conducted this study to compare the hla-dqb1 allelic sequence variation among sle patients and controls in the northeast of iran. genomic dna of 40 sle patients and 83 healthy controls were amplified by polymerase chain react...