an experiment was conducted to investigate the effect of different types of ovarian cycles on reproductive indices and blood metabolites in 61 holstein cows. blood samples were taken from each cow between day 12 and day 50 postpartum (pp). serum progesterone concentrations were measured to determine the onset of luteal function and different types of luteal dysfunctions in cows. serum glucose, ...

The aim of this study was to investigate the effects of ethanolic extract of the whole plant of Phyllanthus rheedii wight P.rheedii as antihyperglycemic, antihyperlipidemic and antioxidant effect in streptozotocin (STZ) induced diabetic rats. Male Wistar rats were administered P.rheedii (250 mg/kg) orally for 21 days and blood glucose level was measured weekly. At the end of 21 days, the serum ...

While vitamin D3 is recognized as a neuroactive steroid affecting both brain development and function, efficient analytical method in determining vitamin D3 metabolites in the brain tissue is still lacking, and the relationship of vitamin D3 status between serum and brain remains elusive. Therefore, we developed a novel analysis method by using high performance liquid chromatography-tandem mass...

Nitric oxide (NO) plays an important role in physiological regulation of the airways. The monitoring of airway inflammation has being observed in bronchial asthma directly, by sputum examination, and indirectly, by measurements in peripheral blood. To investigate the diagnostic value of these two methods, we compared NO metabolites in induced sputum and serum obtained in patients with asthma an...

Environmental enteric dysfunction, an asymptomatic condition characterized by inflammation of the small bowel mucosa, villous atrophy, malabsorption, and increased intestinal permeability, is a major contributor to childhood stunting in low-income countries. Here we report the relationship of increased intestinal permeability with serum metabolites in 315 children without acute malnutrition, ag...

The effect of mannan oligosaccharides (MOS) on health, growth performance, fatty acids deposition, serum, and urine metabolites, as well the correlation between differential metabolites other indexes, were investigated in Hu lambs. In total, 30 seven-day-old male lambs fed a milk replacer with or without 0.2% MOS (15 each). placed this diet until they 28 days old. significantly increased appare...

A current challenge in metabolomics is the reliable quantitation of many metabolites. Limited resolution and sensitivity combined with the challenges associated with unknown metabolite identification have restricted both the number and the quantitative accuracy of blood metabolites. Focused on alleviating this bottleneck in NMR-based metabolomics, investigations of pooled human serum combining ...

In this study ultra performance liquid chromatography (UPLC) coupled to time-of-flight mass spectrometry in the MS mode was used for rapid and comprehensive analysis of metabolites in the serum of mice exposed to internal exposure by Cesium-137 (Cs). The effects of exposure to Cs were studied at several time points after injection of CsCl in mice. Over 1800 spectral features were detected in th...

AIM Nitric oxide (NO) plays a crucial role in vascular tone regulation and is involved in pathogenesis of periodontitis. In this cross-sectional study, we investigated the serum and saliva levels of NO metabolites in periodontal disease and their relationship with serum C-reactive protein (CRP) levels, lipids metabolism and periodontal disease severity. MATERIAL AND METHODS Serum and saliva w...

In this study ultra performance liquid chromatography (UPLC) coupled to time-of-flight mass spectrometry in the MS(E) mode was used for rapid and comprehensive analysis of metabolites in the serum of mice exposed to internal exposure by Cesium-137 ((137)Cs). The effects of exposure to (137)Cs were studied at several time points after injection of (137)CsCl in mice. Over 1800 spectral features w...