We compared the effectiveness of three PCR protocols for the detection of Bifidobacterium adolescentis and one PCR protocol for detecting Bacteroidales as indicators of human fecal pollution in environmental samples. Quantitative PCR indicated that a higher concentration of B. adolescentis DNA was recovered from sewage samples on the 0.2 mum filters compared to the 0.45 mum filters, and there w...

Animal and human wastes contain fecal bacteria, including pathogens that can contaminate groundwater, streams, lakes, and reservoirs through runoff and infiltration. Bacterial nonpoint sources of pollution continually impair water quality (Hartel et al., 2002). These pollution sources may come from failed septic systems, large animal operations, land application of wastes, sewage treatment faci...

Great attention has been paid to the potential of wastewater irrigation as a sustainable water source, particularly due to water scarcity and water pollution issues. However, few studies have focused on its adverse effects and on the health risks it may pose. In this study, the physicochemical properties of soils and plants irrigated with rural domestic wastewater and associated microbiological...

Regulatory requirements – Control of pollution in receiving waters is the main reason for regulation. Many jurisdictions are moving towards regulations prohibiting discharges of untreated sewage into water bodies, and this is a problem for many older systems that carry combined stormwater and sanitary sewage. Even the quality of urban stormwater is coming under scrutiny and limits are being imp...

Anthropogenic environmental changes can serve as drivers for evolutionary responses in wild populations. To predict the long-term impact of anthropogenic changes on populations, it is crucial to understand the genetic effects caused by these disturbances. The Baltic Sea is considered to be one of the world’s most contaminated seas, and the increase of anthropogenic chemical pollution is a major...

Sewage pollution is an increasing problem in tropical marine environments. In this revlew we synthesize present knowledge of the effects of sewage pollution on coral-reef communities, and suggest directions for future research. A wide range of sewage impacts on coral-reef communities has been reported. Little or no impact has been observed on some reefs in well-flushed waters that receive small...

We previously isolated and characterized an Enterococcus faecalis ATCC 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. Here, we report the draft genome sequence of this bacteriophage.