Activation of phagocyte NADPH oxidase requires interaction between p47 and p22. p47 in resting phagocytes does not bind p22. Phosphorylation of serines in the p47 C terminus enables binding to the p22 C terminus by inducing a conformational change in p47 that unmasks the SH3A domain. We report that an arginine/lysine-rich region in the p47 C terminus binds the p47 SH3 domains expressed in tande...

Streptococcus suis serotype 2 (S. suis 2) is a zoonotic pathogen that exhibits high-level resistance and multi-drug resistance to classic antibiotics and causes serious human casualties and heavy economic losses in the swine industry worldwide. Therefore, alternative therapies or novel antibacterial agents need to be developed to combat this pathogen. A novel endolysin derived from the S. suis ...

Neurotransmission involves the exo-endocytic cycling of synaptic vesicle (SV) membranes. Endocytic membrane retrieval and clathrin-mediated SV reformation require curvature-sensing and membrane-bending BAR domain proteins such as endophilin A. While their ability to sense and stabilize curved membranes facilitates membrane recruitment of BAR domain proteins, the precise mechanisms by which they...

Neurotransmission involves the exo-endocytic cycling of synaptic vesicle (SV) membranes. Endocytic membrane retrieval and clathrin-mediated SV reformation require curvature-sensing and membrane-bending BAR domain proteins such as endophilin A. While their ability to sense and stabilize curved membranes facilitates membrane recruitment of BAR domain proteins, the precise mechanisms by which they...

Activation of phagocyte NADPH oxidase requires interaction between p47(phox) and p22(phox). p47(phox) in resting phagocytes does not bind p22(phox). Phosphorylation of serines in the p47(phox) C terminus enables binding to the p22(phox) C terminus by inducing a conformational change in p47(phox) that unmasks the SH3A domain. We report that an arginine/lysine-rich region in the p47(phox) C termi...

The lysin LysGH15, which is derived from the staphylococcal phage GH15, demonstrates a wide lytic spectrum and strong lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). Here, we find that the lytic activity of the full-length LysGH15 and its CHAP domain is dependent on calcium ions. To elucidate the molecular mechanism, the structures of three individual domains of LysGH...

21 P128 is an anti-staphylococcal protein consisting of Staphylococcus aureus phage K derived tail 22 associated muralytic enzyme (TAME) catalytic domain (Lys16) fused with cell wall binding 23 SH3b domain of lysostaphin. In order to understand the mechanism of action and emergence of 24 resistance to P128, we isolated mutants of Staphylococcus spp, including MRSA, resistant to 25 P128. In addi...

P128 is an anti-staphylococcal protein consisting of the Staphylococcus aureus phage-K-derived tail-associated muralytic enzyme (TAME) catalytic domain (Lys16) fused with the cell-wall-binding SH3b domain of lysostaphin. In order to understand the mechanism of action and emergence of resistance to P128, we isolated mutants of Staphylococcus spp., including meticillin-resistant Staphylococcus au...

NOX (NADPH oxidase) plays an important role during several pathologies because it produces the superoxide anion (O2•-), which reacts with NO (nitric oxide), diminishing its vasodilator effect. Although different isoforms of NOX are expressed in ECs (endothelial cells) of blood vessels, the NOX2 isoform has been considered the principal therapeutic target for vascular diseases because it can be ...

The chemistry of group-5 transition metals, namely vanadium, niobium, and tantalum supported by thiolate ligand framework, has been described. Special emphasis given to the electronic structural properties binuclear complexes metals exhibiting interesting coordination modes chelating ligands. mild thermolysis [(CH2S2)4B]Na5 with [Cp*VCl2]3 afforded divanadium trithiocarbonate complex, [(Cp*V)2(...