Molecular techniques based on the polymerase chain reaction (PCR) can provide rapid and sensitive diagnosis of plum pox virus (PPV), the causal agent of the devastating 'sharka' disease of stone fruit trees. The present study compared routine polymerase chain reaction (PCR) procedures against a new system, PCR-ELISA (Boehringer Mannheim), which enables immunoenzymatic detection of PCR products....

Two hуperspectral remote sensing techniques, spectral reflectance and chlorophyll fluorescence, were used for the identification of biotic stress (sharka disease) in plum trees at an early stage without visible symptoms on the leaves. The research was focused on cultivars that are widely spread in Bulgaria: ‘Angelina’, ‘Black Diamond’ and ‘Mirabelle’. Hyperspectral reflectance and fluorescence ...

Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are qu...

plum pox virus (ppv), the causal agent of sharka disease, belongs to the genus potyvirus in the family potyviridae and has been reported from some regions of iran. surveys were conducted to determine the incidence of the virus in khorasan razavi province in 2010 and 2011 and 553 samples of stone fruit trees were collected and tested by das-elisa. in this method 12 samples were infected with ppv...

In this work, a recombinant plum pox virus (PPV, Sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. Alaska). PPV had produced chlorotic spots as well as necrotic spots in the oldest leaves at 13-15 d post-inoculation. At 15 d post-inoculation, PPV was present in the chlorotic and necrot...