The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (...

Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...

The roots and stems of dicotyledonous plants thicken by the cell proliferation in the cambium. Cambial proliferation changes in response to environmental factors; however, the molecular mechanisms that regulate cambial activity are largely unknown. The quadruple Arabidopsis thaliana mutant atipt1;3;5;7, in which 4 genes encoding cytokinin biosynthetic isopentenyltransferases are disrupted by T-...

In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryogenesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tilia platyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentr...

A rapid in vitro propagation system has been established from mature shoot tip and nodal segments of ahighly valuable medicinal plant Alternanthera sessilis (L.). The explants were cultured on Murashige andSkoog’s medium augmented with different concentrations and combinations of plant growth regulators forshoot bud initiation and multiplications. For shoot tip, highest freque...

The influence of two carbon sources, sorbitol and sucrose on In vitro shoot proliferation and rooting of peach rootstock GF 677 was compared at 15, 30, 45 and 60 g l concentrations. The highest number of shoots per proliferated explant, usable shoots and shoots having maximum fresh weight were obtained on sorbitol at 30 g l. GF 677 does not seem to be an efficient utilizer of sucrose for In vit...