signal amplification

abbas ebrahimi- kalan department of neurosciences, faculty of advanced medical sciences, tabriz university of medical sciences, tabriz, iran shahram ahmadian clinical laboratory, imam reza hospital, tabriz university of medical sciences, tabriz, iran hamid tayefi-nasrabadi department of anatomical, faculty of medicine, tabriz university of medical sciences, tabriz, mohammad pourhassan-moghaddam department of medical biotechnology, faculty of advanced medical sciences, tabriz university of medical sciences, tabriz, iran c ian wark research institute, mawson lakes campus, university of south australia, mawson lakes,

introduction: telomeric repeat amplification protocol assay (trap assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. it includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of pcr insid...

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Signal amplification by rolling circle amplification on DNA microarrays.

Journal: :Nucleic acids research 2001

G Nallur C Luo L Fang S Cooley V Dave J Lambert K Kukanskis S Kingsmore R Lasken B Schweitzer

While microarrays hold considerable promise in large-scale biology on account of their massively parallel analytical nature, there is a need for compatible signal amplification procedures to increase sensitivity without loss of multiplexing. Rolling circle amplification (RCA) is a molecular amplification method with the unique property of product localization. This report describes the applicat...

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