Chromosomal appearance of 12 Holstein bulls selected for artificial inseminations were examined bysister chromatid exchanges (SCEs). For differential staining of sister chromatids bromodeoxyuridine (BrdU)was inoculated in lymphocyte cultures. The mean, maximum and minimum number of SCE per cell weredetermined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. SCE frequencies of all animals were...

We have scored frequency of transformation and appearance of the oncogenic marker "long microvilli" in mouse embryo fibroblast C3H/10T 1/2 cells in culture for the compound Ni3S2. Furthermore, we have scored for Ni3S2-induced sister chromatid exchange in cultured human lymphocytes. Nickel subsulfide in moderate doses caused morphological transformation to type I, II, and III foci and induced lo...

A study was performed to evaluate the effect on the frequency of sister chromatid exchange in first trimester chorionic villi of two chemical compounds, mitomycin C and cyclophosphamide. Mitomycin C is generally known to induce an increase in sister chromatid exchange both in vivo and in vitro standard conditions. Cyclophosphamide is a compound requiring additional enzymatic enrichment of the c...

Polyamines have been implicated in the intracellular stabilization of DNA. Depletion of intracellular polyamines influences the cytotoxicity of 1,3-bis(2-chloroethyl)-1-nitrosourea and cis-diamminedichloroplatinum II. By means of the sister chromatid exchange assay, it was found that intracellular polyamine depletion can also alter the induction of chromosomal damage by these cytotoxic agents.

We have scored frequency of transformation and appear ance of the oncogenic marker "long microvilli" in mouse em bryo fibroblast C3H/10TV2 cells in culture for the compound NI3S2. Furthermore, we have scored for Ni3S2-induced sister chromatid exchange in cultured human lymphocytes. Nickel subsulfide in moderate doses caused morphological transfor mation to type I, II, and III foci and induced l...

The frequency of spontaneous sister chromatid exchange was studied in normal marrow derived from 38 healthy donors and 40 untreated patients with chronic phase CML. The sister chromatid exchange frequency was significantly lower in the leukemic cells (range, 2.32 +/- 1.31 to 4.76 +/- 2.37 per metaphase; mean, 3.18 +/- 0.49) than in normal marrow (range, 2.36 +/- 1.44 to 5.54 +/- 2.24 per metaph...

A positive selection system for intrachromosomal recombination in Saccharomyces cerevisiae has been developed. This was achieved by integration of a plasmid containing an internal fragment of the HIS3 gene into its chromosomal location. This resulted in two copies of the HIS3 gene one with a terminal deletion at the 3' end and the other with a terminal deletion at the 5' end. Reversion of the g...

Sister chromatids are often arranged as incompletely aligned entities in interphase nuclei of Arabidopsis thaliana. The STRUCTURAL MAINTENANCE OF CHROMOSOMES (SMC) 5/6 complex, together with cohesin, is involved in double-strand break (DSB) repair by sister chromatid recombination in yeasts and mammals. Here, we analyzed the function of genes in Arabidopsis. The wild-type allele of SMC5 is esse...