Mutagenesis is an important tool for breeding and genomic research. In this study, the germinated seeds isolated microspores of a double haploid line ‘FT’ were treated with EMS, respectively, aim comparing effects two approaches on generating mutants in Chinese cabbage. For microspore EMS mutagenesis, 0.12% 20 min, total 1268 plantlets obtained, 15 M1 screened mutation frequency 1.2%. seed 7800...

a pseudomonas aeruginosa mutant derived by random mutagenesis with n-methyl-n¢-nitro-n-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on siegmund-wagner (sw) plates. the mutant designated p. aeruginosa ptcc1637 produces rhamnolipids at concentrations 10 time more than parent strain. nmr analysis and surface tension measurement showed that the biosurfactan...

We have obtained a complete set of 20 variants of the a subunit of tryptophan synthase of Escherichia coli at position 49 in order to extend our previous studies on the effects of single amino acid replacements at position 49 on structure and function. Thirteen mutant a subunits have been newly constructed by sitedirected mutagenesis using oligonucleotides. Six mutants were available from previ...

Nanochemistry is the synthesis and study of well-defined structures with dimensions of 1 ± 100 nanometers (nm), and thus spans the size range between molecules and materials.[1] While supramolecular chemistry (making small molecules bigger) and microfabrication techniques (making big structures smaller) attack from the flanks, biology employs many constructs of this size. Examples include the p...

It is known that p53 alterations are commonly found in tumour cells. Another marker of tumorigenesis is FAK (focal adhesion kinase), a non-receptor kinase that is overexpressed in many types of tumours. Previously we determined that the N-terminal domain of FAK physically interacted with the N-terminal domain of p53. In the present study, using phage display, sitedirected mutagenesis, pulldown ...

Polycomb proteins represent epigenetic regulators and exert transcriptional repression via polycomb repressive complexes (PRC). EZH2 is a component of PRC2, where it functions as a lysine methyl transferase, particularly for histones. In cancer, EZH2 is found to be overexpressed, and in glioblastoma multiforme, EZH2 has been shown to be important for maintenance of glioma stem cells (GSC). Alth...

Polycomb proteins represent epigenetic regulators and exert transcriptional repression via polycomb repressive complexes (PRC). EZH2 is a component of PRC2, where it functions as a lysine methyl transferase, particularly for histones. In cancer, EZH2 is found to be overexpressed, and in glioblastoma multiforme, EZH2 has been shown to be important for maintenance of glioma stem cells (GSC). Alth...

Polycomb proteins represent epigenetic regulators and exert transcriptional repression via polycomb repressive complexes (PRC). EZH2 is a component of PRC2, where it functions as a lysine methyl transferase, particularly for histones. In cancer, EZH2 is found to be overexpressed, and in glioblastoma multiforme, EZH2 has been shown to be important for maintenance of glioma stem cells (GSC). Alth...

background: finding shows that there were several problems in the treatment of helicobacter pylori infection such as the emergence of resistance to the antibiotics, the risk of recrudescence, and the high cost of treatment. the ineffectiveness of conventional treatment mechanisms against cancer cells reveals the importance of peptides as a novel therapeutic approach. however, the short length o...