A theoretical background is provided for the methods of the analysis and topological separation of complex macrocyclic polymers by means of size exclusion chromatography (SEC), liquid adsorption chromatography, and liquid chromatography at the critical condition. We focus on a particular problem of the separation of theta-shaped polymers from its three-armed star analogues. Based on the theory,...

Acute leukemia is a disease pathologically manifested at both genomic and proteomic levels. Molecular genetic technologies are currently widely used in clinical research. In contrast, sensitive and high-throughput proteomic techniques for performing protein analyses in patient samples are still lacking. Here, we used a technology based on size exclusion chromatography followed by immunoprecipit...

Abbreviations: FVIII/vWF: Complex of Factor VIII and von Willebrand Factor; MW: Molecular Weight; NDF: Number of Degree of Freedom; S/D: Solvent/Detergent; LEV/LEVs: Virus (es) Enveloped by Lipid Membrane; NLEV/NLEVs: Non-enveloped Virus (es); IFA method: Method of Immune-fermental Analysis; FITC: Fluorescein Isothiocyanate; RT-PCR method: Real-time PCR Method; TCID50: 50% Tissue Culture Infect...

The Pentaneand Toluene-Soluble Fractions of a Petroleum Residue and three Coal Tars by Size Exclusion Chromatography and UV-Fluorescence Spectroscopy — A petroleum atmospheric pressure distillate residue and three tars derived from different coals using different severities of thermal treatment were separated into seven fractions using column chromatography on silica and sequential elution by t...

Proteins and other macromolecules of interest can be purified from crude extracts or other complex mixtures by a variety of methods. Selective precipitation is perhaps the simplest method for separating one type of macromolecule from another. Most purification methods, however, involve some form of chromatography whereby molecules in solution (mobile phase) are separated based on differences in...

The first implementation and use of an in situ size exclusion chromatography (SEC) system on a small-angle neutron scattering instrument (SANS) is described. The possibility of deploying such a system for biological solution scattering at the Institut Laue-Langevin (ILL) has arisen from the fact that current day SANS instruments at ILL now allow datasets to be acquired using small sample volume...

The effect of solvent power and flow rate on the permeability of a size exclusion chromatography (SEC) column packed with a novel polyvinylpyrrolidone (PVP)-grafted porous silica resin was studied. The PVP-silica resin was prepared from a porous silica size exclusion resin onto which a terminally anchored PVP layer was graft polymerized. The terminally anchored PVP layer was synthesized via a f...

Abbreviations: mAbs: Monoclonal Antibodies; CQA: Critical Quality Attribute; CDRs: Complementary Determining Regions; FC: Fragment Crystallizable; FAB: Fragment Antigen-Binding; SEC: Size-Exclusion Chromatography; SDS-PAGE: Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis; CE: Capillary Electrophoresis; IEX: Ion Exchange Chromatography; RPC: Reversed Phase Chromatography; MS: Mass Spect...

Size-exclusion chromatography (SEC) and capillary zone electrophoresis (CZE) coupled with inductively coupled plasma mass spectrometry were applied to characterize low, medium, and high molecular weight cadmium complexes with glutathione and phytochelatins (PCs). The dominant stoichiometry of the complexes formed in vitro was established as 1:1 using electrospray ionization mass spectrometry. C...

Metals are essential for protein function as cofactors to catalyze chemical reactions. Disruption of metal homeostasis is implicated in a number of diseases including Alzheimer's and Parkinson's disease, but the exact role these metals play is yet to be fully elucidated. Identification of metalloproteins encounters many challenges and difficulties. Here we report an approach that allows metallo...