In this work we follow the polyethylcyanoacrylate nanoparticles through human skin. Photoluminescence (PL) is used to quantify the amount of these nanocarriers into the layers of the skin stratum corneum (SC). To obtain samples of each layer of SC, tape stripping technique was conducted. Reproducible data were obtained, and the nanoparticles’ distribution in each layer of the SC is presented. P...

Atopic dermatitis (AD) is characterized by epidermal tight junction (TJ) defects and a propensity for Staphylococcus aureus skin infections. S. aureus is sensed by many pattern recognition receptors, including Toll-like receptor 2 (TLR2). We hypothesized that an effective innate immune response will include skin barrier repair, and that this response is impaired in AD subjects. S. aureus-derive...

The correlation between skin barrier function and transepidermal water loss (TEWL) was evaluated in dogs. Stratum corneum (SC) of 10 healthy dogs was removed by tape stripping (TS), which decreased the corneal layer to allow for permeation of fluorescent dye into skin. TEWL of damaged skin was measured with the closed-chamber-type TEWL analyzer, CC-01. The frequency of TS was directly related t...

Objective With rapid development of nanotechnology, there is increasing interest in nanoparticle (NP) application and its safety and efficacy on human skin. In this study, we utilized confocal laser scanning microscopy to estimate NP skin penetration. Methods Three different-sized polystyrene NPs marked with red fluorescence were applied to human skin, and Calcium Green 5N was used as a count...

Surgical wounds generally heal by primary intention with few complications (Ousey et al, 2011). However, certain patient factors and surgical types may predispose postoperative incisions to skin blistering, particularly if an unsuitable postoperative wound dressing is used. Skin blistering occurs when the epidermis becomes separated from the dermis and results from continued friction on the ski...

Wounding by incision was a promoting stimulus in mouse skin previously initiated with 7,12-dimethylbenz-(a)anthracene. Skin massage elicited a marked proliferative response in skin but was not a promoting stimulus. Wounding mouse skin, either by multiple scalpel incisions or by stripping with silicon carbide paper, led to a marked induction of ornithine decarboxylase activity. In both instances...

Unspecified DOI: 10.1016/j.jaci.2014.07.037 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: http://doi.org/10.5167/uzh-108857 Originally published at: von Moos, Seraina; Johansen, Pål; Tay, Fabian; Graf, Nicole; Kündig, Thomas M; Senti, Gabriela (2014). Comparing safety of abrasion and tape-stripping as skin preparation in allergen-specific epicutaneous immunoth...

The aim of this study was to investigate the amount of protein in stratum corneum in atopic dermatitis (AD) patients and healthy controls, using tape stripping technique. Furthermore, to compare two different methods for protein assessment. Tape stripping was performed in AD patients and healthy controls to collect stratum corneum samples and subsequently analysed with two different methods: Sq...

Speciation modeling is an essential tool to estimate the chemical composition of an aqueous sample and to assess the bioavailability and potential risks associated with metal contamination. It is also an indispensable alternative for quantifying the metal speciation when analytical techniques are not available. In this study, we tested the effectiveness of two models, WinHumicV and the Non-Idea...