نتایج جستجو برای: small interfering rna

تعداد نتایج: 1014115  

Journal: :Records of Pharmaceutical and Biomedical Sciences 2022

مهاجری, نسرین , کاظمی, بهرام,

RNA interference) RNAi) که ازسوی siRNA) Small interfering RNA) میانجیگری می شود، ابزار قدرتمندی برای مهار بیان ژن در رده های مختلف سلولی است. siRNA ها علاوه بر عمل خاموش کردن ژن در القای سایتوکاین های پیش التهابی (Pro-inflammatory) و اینترفرون نوع I نیز دخالت دارند. گیرنده های ایمنی شناسایی کننده RNA شامل ( Toll-like receptor (TLR ها و پروتئین های سیتوزولی شناساگر RNA مانند: PKR1, RIG-I 2, MDA-...

Journal: :Biomolecular engineering 2004
Peter Hahn Cornelia Schmidt Martin Weber Jie Kang Wolfgang Bielke

mRNA targeted by siRNA is endogeneously cleaved into a 5'- and a 3'-fragment and finally degraded in cells. Little is known about the relative stability and degradation kinetics of these 5'- and 3'-fragments after the siRNA mediated first cut. We present a qRT-PCR protocol which allows the determination of the optimal time point for mRNA analyses, helping to avoid the generation of false positi...

Journal: :RNA 2006
Xiang Liu Feng Jiang Savitha Kalidas Dean Smith Qinghua Liu

In Drosophila melanogaster, the Dicer-2/R2D2 complex initiates RNA interference (RNAi) by processing long double-stranded RNA (dsRNA) into small interfering RNA (siRNA). Recent biochemical studies suggest that the Dcr-2/R2D2 complex also facilitates incorporation of siRNA into the RNA-induced silencing complex (siRISC). Here we present genetic evidence that R2D2 and Dcr-2 are both required for ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2003
Andrew Dillin

T he discovery of transgene silencing in plants and doublestranded RNA (dsRNA) interference in the worm Caenorhabditis elegans has led to the latest revolution in molecular biology, RNA interference (RNAi). Over 10 years ago it was noted that several transgenic plant lines each containing the same ectopic transgene not only failed to be expressed but also inhibited the expression of the endogen...

Journal: :Chembiochem : a European journal of chemical biology 2001
T Tuschl

The term aRNA interferenceo (RNAi) was coined after the groundbreaking discovery that injection of double-stranded RNA (dsRNA) into the nematode Caenorhabditis elegans leads to specific silencing of genes highly homologous in sequence to the delivered dsRNA. The RNAi phenotype is either identical to the genetic null mutant or resembles an allelic series of mutants. The dsRNA can also be deliver...

Journal: :Haematologica 2010
Michael Koldehoff Lambros Kordelas Dietrich W Beelen Ahmet H Elmaagacli

BACKGROUND Selective inhibition of the BCR-ABL tyrosine kinase by RNA interference has been demonstrated in leukemic cells. We, therefore, evaluated specific BCR-ABL small interfering RNA silencing in BCR-ABL-positive cell lines, including those resistant to imatinib and particularly those with the T315I mutation. DESIGN AND METHODS The factor-independent 32Dp210 BCR-ABL oligoclonal cell line...

Journal: :Advanced drug delivery reviews 2009
Donald D Rao John S Vorhies Neil Senzer John Nemunaitis

RNA interference (RNAi) is a natural process through which expression of a targeted gene can be knocked down with high specificity and selectivity. Using available technology and bioinformatics investigators will soon be able to identify relevant bio molecular tumor network hubs as potential key targets for knockdown approaches. Methods of mediating the RNAi effect involve small interfering RNA...

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