The oligo microarray (DNA chip) technology in recent years has a significant impact on genomic study. Many fields such as gene discovery, drug discovery, toxicological research and disease diagnosis, will certainly benefit from its use. A microarray is an orderly arrangement of thousands of DNA fragments where each DNA fragment is a probe (or a fingerprint) of a gene/cDNA. It is important that ...

As researchers delve deeper into the human genome, demand for highly sensitive and reproducible RNA-seq is growing. Next-generation sequencing (NGS) applications offer diverse and detailed analysis for these studies, but manual library preparation is repetitive, tedious, and prone to human error and variability. In contrast to manual preparation methods, the SMARTer Apollo system brings the ben...

A label-free-based fiber optic biosensor based on etched tilted Bragg grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe with a tilt angle of 10°, while the etching accomplished hydrofluoric acid. composite polyethylenimine (PEI)/poly(acrylic acid) (PAA) thermally deposited TFBG, followed by immobilization probe DNA (pDNA) this...

Cystatins are natural tight-binding reversible inhibitors of cysteine proteases. In this study, a cDNA library was constructed from Collichthys lucidus using the SMART technique. A complete cDNA sequence with high identity to the conserved sequence of the cystatin C gene was cloned from the library using EST analysis and rapid amplification of cDNA ends (RACE), then subjected to further investi...

objective(s): hypoxia is a serious challenge for treatment of solid tumors. this condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (who) astrocytoma grade iv. hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. furthermore, in molecular level, hypoxia can cause induction of dna brea...

existed electricity grids deliver produced power to the consumer passing through transmission and distribution grids. according to high losses of these grids in transmission level and inexistence of bilateral interaction for simultaneous information exchange, a concept of smart grids were made by capabilities such as consciously participation of consumers in the smart electricity grids, an amou...

pigeonpea (cajanus cajan (l) millsp.) is a drought tolerant legume widely grown in the arid and semi-arid tropics of the world which possesses a deep and extensive root system that succors a number of important physiological and metabolic functions to cope with drought. application of available functional genomics approaches to improve productivity under water deficit requires a better understa...

background: cholera is a potentially epidemic and life-threatening secretory diarrhoea characterized by voluminous watery stools, often accompanied by vomiting, and resulting in hypovolemic shock and acidosis. it is caused by certain strains of the species vibrio cholerae which can also cause mild or in apparent infections. the aim of this study is the evaluation of capsaicin, as a potential in...