sewage water by land filters that nitrification Was due to microbial activity, and since then an increasing amount of attention has been devoted to the investigation of micro-organisms in relation to sewage purification and to the closely allied processes of soil metabolism. A considerable volume of literature has accumulated on the microbiology of these processes, but there is need for further...

AIMS In this study, a loop-mediated isothermal amplification (LAMP) assay has been developed and evaluated for the rapid and sensitive detection of Verticillium dahliae Kleb., the causal agent of vascular wilts in many economically important crops. METHODS AND RESULTS LAMP primers were designed based on a previously described RAPD marker, and the LAMP assay was applied for direct detection of...

In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly critical for some microorganisms which resist classical techniques, we used sma...

In recent years, several protocols based on the extraction of nucleic acids directly from the soil matrix after lysis treatment have been developed for the detection of microorganisms in soil. Extraction efficiency has often been evaluated based on the recovery of a specific gene sequence from an organism inoculated into the soil. The aim of the present investigation was to improve the extracti...

Explanation............................................................................... 8 Biological data .......................................................................... 9 Biochemical aspects .......................................................... 9 Toxicological studies .......................................................... 11 Cytotoxicity and genotoxicity ...................

Functional Land Management (FLM) is proposed as an integrator for sustainability policies and assesses the functional capacity of the soil and land to deliver primary productivity, water purification and regulation, carbon cycling and storage, habitat for biodiversity and recycling of nutrients. This paper presents the catchment challenge as a method to bridge the gap between science, stakehold...

Results of extensive studies on the basic problems of extraction, isolation, fractionation and purification of soil humic substances are presented. Data on the degradation, functional group analysis and molecular weight determination of purified humic fractions froro the fJ -horizon of a podzol are reported, and the role of huroic substances in the podzolization process is discussed.

During the course of our screening program for new antibiotics, Streptomyces sp. 80432 isolated from a soil sample was found to produce a water-soluble antibiotic. This antibiotic, named minimycin, showed not only inhibitory activity against Grampositive and Gram-negative bacteria, but also antitumor activity. Isolation and purification by a chromatography gave a colorless crystalline substance...

A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange chromatograph...