Background Heat shock protein A2 (HSPA2) is correlated with sperm maturity and function. Therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. On the other hand, DNA damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with DNA fragmentation and chromatin abnormalities in human ejaculates is well documented, ...

Introduction: This study aimed to perform routine seminal fluid analysis, sperm DNA fragmentation, and function tests at the chromatin maturation level evaluate pregnancy in patients passing intrauterine insemination before starting Intrauterine Insemination (IUI) method. Materials Methods: In this prospective study, 111 couples who underwent unexplained infertility were admitted Al-Farah IVF a...

BACKGROUND Methamphetamine (MA) was shown to have harmful effects on male reproductive system. OBJECTIVE To investigate probable effects of daily administration of MA on sperm parameters and chromatin/DNA integrity in mouse. MATERIAL AND METHODS Thirty-five NMRI male mice were divided into five groups including low, medium, and high dosage groups which were injected intraperitoneally with 4...

introduction: tegrity is the most important component for transferring genetic information sperm dna fragmentation is considered a component of male infertility. its assessment comprises conventional semen analysis of which emphasis is placed on its evaluation in fertility clinics. in this study, we investigate the relationship between sperm parameters of motility, morphology, concentration, an...

Objective The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and D...

Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...