introduction: tegrity is the most important component for transferring genetic information sperm dna fragmentation is considered a component of male infertility. its assessment comprises conventional semen analysis of which emphasis is placed on its evaluation in fertility clinics. in this study, we investigate the relationship between sperm parameters of motility, morphology, concentration, an...

Introduction: tegrity is the most important component for transferring genetic information Sperm DNA fragmentation is considered a component of male infertility. Its assessment comprises conventional semen analysis of which emphasis is placed on its evaluation in fertility clinics. In this study, we investigate the relationship between sperm parameters of motility, morphology, concentratio...

BACKGROUND Leukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to detect semen leukocytes but it may be inaccurate. The aims of this study were (i...

BACKGROUND This is the first study to assess the outcome of sperm washing and intrauterine insemination (IUI) cycles in human immunodeficiency virus-positive (HIV(+)) men to determine any predictors of success, as well as evaluating the effect of HIV on sperm parameters. METHODS Semen characteristics were evaluated in 106 HIV(+) men and a control group of 234 HIV(-) men, and the effect of mar...

BACKGROUND Intrauterine insemination (IUI) could become preferred to more invasive and expensive techniques of assisted reproduction therapy (ART) and should be offered as the first choice in cases with no female factors and mild male factor subfertility. However, developing countries and especially their rural areas often lack the necessary equipment and laboratory facilities. OBJECTIVE To d...

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of plati...

The objective of this study was to investigate the influence of various factors on blastocyst development in a bovine in vitro embryo production system. Two production systems were originally compared: Protocol 1, which utilized oocyte recovery by slicing, 20 % fetal bovine serum with FSH (FBS+) for oocyte maturation, and sperm preparation system 1 (F1), and Protocol 2, which utilized oocyte re...