Objective: To compare two suites of culture media (one with HSA and one protein-free (PF) supplemented with methylcellulose) for washing human sperm in IVF. Methods: Semen samples (n = 41) underwent parallel density gradient preparation using PF or HSA-supplemented culture medium and subsequent yield, survival, morphology and motility were compared. Results: The PF medium resulted in a signific...

________________________________________________________________________________ Abstract The study evaluated the effect of different cryoprotectants on post-thaw survival and motility of Kolbroek sperm. Semen from Kolbroek boars was collected with the gloved hand technique. Ejaculates were diluted with Beltsville thawing solution (BTS) at a ratio of 1 : 1 prior to freezing. Semen was diluted w...

Sperm cells are complex models for handling in vitro, their viability is limited, and their physiology is complex. The study of their properties is of great application in the animal production industry, to improve the selection of gametes, control pathologies and for the development of cryobiology protocols. It is therefore important to have viable and functional gametes. Consequently, it has ...

Sperm competition between the ejaculates of multiple males for the fertilization of a given set of ova is taxonomically widespread. Males have evolved remarkable adaptations to increase their reproductive success under postcopulatory sexual selection, which in many species includes the ability to modify behaviour and ejaculate characteristics plastically to match the perceived level of sperm co...

In vitro induced apoptosis by dexamethazone treating of ram spermatozoa provoked the phospatidylserine exposure which is mainly localized on sperm tail region of plasma membrane and is correlated negatively with the sperm motility and survival rate.

Optimal sperm cryopreservation is a prerequisite for the sustainable commercial application of frozen-thawed boar semen for AI. Three experiments were performed to identify factors influencing variability of postthaw sperm survival among 464 boar ejaculates. Sperm-rich ejaculate fractions were cryopre-served using a standard freezing-thawing procedure for 0.5-mL plastic straws and computer-cont...

Fish sperm cryopreservation is considered as a valuable technique for artificial reproduction and genetic improvement (Chao & Liao, 2001; Kopeika et al., 2007; Rana, 1995; Suquet et al., 2000). Semen quality must be monitored when attempts are made to increase the efficiency of artificial fertilization, to cryopreserve only sperm of high quality, and to evaluate frozenthawed sperm. Cryopreserve...

In 2007, the Intergovernmental Panel on Climate Change projected an average global air temperature increase of 1.1-6.4°C by the end of the 21st century. Although the tropics are predicted to experience less extreme temperature increases than regions of higher latitude, tropical ectotherms live close to their thermal limits, and are thus particularly vulnerable to increases in temperature. In th...

Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The p...