The formation of mature spermatozoa is one of the most essential functions in life. A concerted sequence of events is needed to proliferate, maintain and mature germ cells starting with spermatogonial stem cells and culminating in mature gametes. Apart from the genetic background, this process requires highly organized tissue in which the complex process of spermatogenesis is strongly regulated...

The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

Background: The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells have provided a very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules consist of spermatogonial stem cells. To investigate and biomanipulate these cells, first the proliferation and viability rate of cells s...

The use of a transgenic line of rats that express enhanced GFP (EGFP) exclusively in the germ line has allowed a separation of feeder layers and contaminating testis somatic cells from germ cells and the identification of a set of spermatogonial stem cell marker transcripts. With these molecular markers as a guide, we have now devised culture conditions where rat spermatogonial stem cells renew...

introduction: nowadays, spermatogonial stem cells (sscs) cultivation has been used by many researchers as an effective tool for infertility treatments. oxidative conditions can be effective on cell proliferation and differentiation of these cells. so, the aim of this study was to establish oxidative stress model for antioxidant activity of some drugs investigation during sscs in vitro culture. ...

the complex process of spermatogenesis is regulated by various factors. studies on spermatogonial stem cells (sccs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. to investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

CONTEXT Young boys treated with high-dose chemotherapy are often confronted with infertility once they reach adulthood. Cryopreserving testicular tissue before chemotherapy and autotransplantation of spermatogonial stem cells at a later stage could theoretically allow for restoration of fertility. OBJECTIVE To establish in vitro propagation of human spermatogonial stem cells from small testic...

background: in cancer patients, chemo and radiotherapy can cause infertility by damaging spermatogenesis process. this process is based on self-renewal and differentiation of a rare population of the testicular cells called spermatogonial stem cells (sscs). scientists have tried to isolate, enrich and culture human spermatogonial stem cells, hoping to resolve infertility problems in cancer reco...

background and aims: in this lab trial, the effect of scaffold based on human serum albumin (hsa) and hydroxyapatite nanoparticles (ha nps) on mouse spermatogonial cell line (scl) was investigated. materials and methods: to synthesize ha nps, calcium nitrate and diammonium phosphate at ph 13 were gently added and heated at 100 ºc for 24 hours. then serial concentrations of ha nps was separately...

BACKGROUND To isolate and characterization of human spermatogonial stem cells from stem spermatogonium. METHODS The disassociation of spermatogonial stem cells (SSCs) were performed using enzymatic digestion of type I collagenase and trypsin. The SSCs were isolated by using Percoll density gradient centrifugation, followed by differential surface-attachment method. Octamer-4(OCT4)-positive SS...