We investigated the inhibition of Clostridium perfringens spore germination and outgrowth by two green tea extracts with low (green tea leaf powder [GTL]; 141 mg of total catechins per g of green tea extract) and high (green tea leaf extract [GTE]; 697 mg of total catechins per g of extract) catechin levels during abusive chilling of retail cooked ground beef, chicken, and pork. Green tea extra...

Physiological races of a fungal phytopathogen represent biotypes capable of attacking certain varieties of a susceptible host-species. Attempts to distinguish physiological races by other criteria have not yet been successful. On the other hand, strains of bacterial pathogens may often be identified using bacterial viruses (bacteriophage). Attempts to demonstrate a virus of a true fungus have n...

Recently, P. Gerhardt and E. Ribi (J. Bacteriol. 88:1774, 1964) reported that the exosporium of the spore of Bacillus cereus consisted of a nap of hairlike projections and a basal membrane. Similar structures were also seen in B. anthracis spores. This paper reports the ultrastructures of both resting and germinating spores of B. anthracis, particularly the presence of the nap, as revealed by e...

The effects of different sources of carbon, nitrogen and some of trace elements on cultivation of Bacillus thuringiensis H14 were studied. Glucose, glycerol, sodium acetate, (NH4)2SO4, corn steep liquor, yeast extract, hydrolyzed casein, Ca2+, Mg2+ and Mn2+ were the main parameters evaluated by Plackett-Burman statistical method. All el...

The efficacy of different concentrations of aqueous neem leaf extract (3.12 to 50 mg/mL) on growth and citrinin production in three isolates of Penicillium citrinum was investigated under laboratory conditions. Mycotoxin production by the isolates was suppressed, depending on the concentration of the plant extract added to culture media at the time of spore inoculation. Citrinin production in f...

Chronic feeding of carcinogens p-dimethylamino azobenzene (initiator) and phenobarbital (promoter) for 90 and 120 days elevated activities of acid and alkaline phosphatases, levels of blood glucose and cortisol and decreased the activities of glutathione reductase, succinate dehydrogenase, and blood cholesterol and hemoglobin contents, and levels of serum estradiol and testosterone in mice. Lev...

The levels of fatty acids and their distribution were determined in cultures of Bacillus megaterium during growth, sporulation, and germination. Branched-chain pentadecanoates (br-C15) were the principal fatty acids of log-phase cells. Synthesis of branched-chain tetradecanoates (br-C14) during sporulation increased the relative proportion of these branched fatty acids in sporulating cells and ...

Control of anthracnose in green pepper involves the use of resistant varieties and/or fungicides. The selection of varieties and efficient products demands great amounts of conidia as inoculum. It is thus necessary to optimize the production of Colletotrichum gloeosporioides conidia in the laboratory, establishing the best conditions for fungus development. The present study aimed at determinin...

Bacillus amyloliquefaciens NJN-6 produces volatile compounds (VOCs) that inhibit the growth and spore germination of Fusarium oxysporum f. sp. cubense. Among the total of 36 volatile compounds detected, 11 compounds completely inhibited fungal growth. The antifungal activity of these compounds suggested that VOCs can play important roles over short and long distances in the suppression of Fusar...

Spore germination and appressorium formation of Magnaporthe oryzae spores was completely suppressed by an ethyl acetate extract of the culture filtrate from the H921 isolate (H921-EAE-CF). Production of antifungal substance(s) in the H921-EAE-CF began to increase up to 3 days after isolate H921 incubation. Furthermore, heat treatment (105 ̊C or 121 ̊C) of H921-EAE-CF did not alter its inhibitory ...